1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Hemagglutinin
  5. HA/Hemagglutinin 蛋白, H1N1 (P03452, sf9, His)

HA/Hemagglutinin 蛋白, H1N1 (P03452, sf9, His)

目录号: HY-P74034
COA 产品使用指南

血凝素蛋白 (HA) 是一种 I 类病毒融合蛋白,可与含唾液酸的受体结合并启动病毒附着于细胞。这种结合通过各种内吞途径促进病毒粒子的内化。HA/Hemagglutinin 蛋白, H1N1 (P03452, sf9, His) 是重组的 HA/血凝素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

Hemagglutinin protein (HA) is a class I viral fusion protein that binds to sialic acid-containing receptors and initiates viral attachment to cells. This binding promotes internalization of virions through various endocytic pathways. HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

血凝素蛋白 (HA) 是一种 I 类病毒融合蛋白,与细胞表面上含唾液酸的受体结合,促进病毒颗粒附着到细胞上。这种附着导致病毒粒子通过网格蛋白依赖性内吞作用或网格蛋白和小窝蛋白独立途径内化。HA 在确定病毒的宿主范围限制和毒力方面也起着至关重要的作用。通过介导内吞病毒颗粒膜与内体膜的融合,HA 使病毒能够渗透到细胞质中。在内体的低pH环境下,HA2发生不可逆的构象变化,释放融合疏水肽并形成有能力的融合孔。HA 作为由二硫键连接的 HA1-HA2 亚基组成的同源三聚体存在,并与人 CACNA1C 相互作用。

生物活性

Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.02-0.4 μg/mL for 1% GRBC.

种属

Virus

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P03452 (D18-Q528)

基因 ID

956529  [NCBI]

蛋白结构
N-term
H1N1 HA (M1-Q528)
Accession # P03452
His
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin Protein, H1N1 (A/Puerto Rico/8/1934, sf9, His)
分子量

Approximately 59.2 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HA/Hemagglutinin Protein, H1N1 (P03452, sf9, His)
目录号:
HY-P74034
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