2. 重组蛋白
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His)

HA/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His)

目录号: HY-P74033
产品使用指南

HA/血凝素蛋白结合细胞表面的受体，促进病毒附着。它通过内吞作用导致内化。HA 决定宿主范围和毒力，介导病毒和内体膜的融合。HA2在内体中发生构象变化，释放融合肽并形成融合孔。孔的形成需要多个HA三聚体。HA/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His) 是重组的 HA/血凝素蛋白，由 HEK293 表达，带有 C-His 标签。HA/Hemagglutinin 蛋白, H1N1 (P03453, HEK293, His) 全长 344 个氨基酸，分子量约为 ~38.3 kDa。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The HA/Hemagglutinin protein binds receptors on the cell surface, facilitating virus attachment. It leads to internalization via endocytosis. HA determines host range and virulence, mediating fusion of virus and endosomal membrane. HA2 undergoes conformational change in endosomes, releasing fusion peptide and forming fusion pore. Multiple HA trimers are required for pore formation. HA/Hemagglutinin Protein, H1N1 (P03453, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293, with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H1N1 (P03453, HEK293, His) is 344 a.a., with molecular weight of ~38.3 kDa.
研究背景

HA/血凝素蛋白与细胞表面上含唾液酸的受体结合，促进病毒颗粒附着到细胞上。这种附着导致大约三分之二的病毒颗粒通过网格蛋白依赖性内吞作用内化，而其余三分之一遵循网格蛋白和小窝蛋白独立途径。该蛋白质在确定宿主范围限制和毒力方面起着至关重要的作用。作为I类病毒融合蛋白，它通过介导内吞病毒颗粒膜与内体膜的融合，负责病毒渗透到细胞质中。在内体的低pH环境下，HA2发生不可逆的构象变化，导致融合疏水肽的释放并形成有能力的融合孔。HA 的多个三聚体对于该孔的形成是必需的。
种属

Virus
表达系统

HEK293
标签

C-His
蛋白编号

P03453 (M1-R344)
基因 ID

/
蛋白结构
N-term
H1N1 HA (M1-R344)
Accession # P03453
His
C-term
同用名
HA; Hemagglutinin; HA/Hemagglutinin Protein, H1N1 (A/USSR/90/1977, HEK293, His)
分子量

Approximately 38.3 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HA/Hemagglutinin Protein, H1N1 (P03453, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
HA/Hemagglutinin Protein, H1N1 (P03453, HEK293, His)
目录号：
HY-P74033
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z