HA/Hemagglutinin 蛋白, H5N1 (H8PF45, HA1, HEK293, His)

HA/Hemagglutinin Protein is pivotal in the viral life cycle, binding to sialic acid receptors and facilitating virus attachment to host cells. This interaction prompts virion internalization through clathrin-dependent or -independent pathways. As a Class I viral fusion protein, it mediates fusion between the virus particle and endosomal membrane, allowing cytoplasmic entry. In endosomes, the low pH induces an irreversible conformational change in HA2, releasing a fusion hydrophobic peptide. Multiple HA protein trimers are essential for forming a competent fusion pore. Its roles in receptor binding, viral internalization, fusion, and membrane penetration are crucial in determining host range restriction and virulence. HA/Hemagglutinin Protein, H5N1 (H8PF45, HA1, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293, with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H5N1 (H8PF45, HA1, HEK293, His) is 340 a.a., with molecular weight of ~38.1 kDa.

HA 蛋白，也称为血凝素蛋白，在病毒生命周期中起着至关重要的作用。它与含有唾液酸的细胞表面受体结合，促进病毒颗粒附着到宿主细胞上。这种附着导致病毒粒子通过网格蛋白依赖性内吞作用途径或网格蛋白和小窝蛋白独立途径内化。HA蛋白是I类病毒融合蛋白，负责介导内吞病毒颗粒膜与内体膜的融合。这种融合事件允许病毒进入细胞的细胞质。内体中的低pH环境会触发蛋白质HA2亚基的不可逆构象变化，导致融合疏水肽的释放。HA 蛋白的多个三聚体对于形成有效的融合孔是必需的。HA蛋白在受体结合、病毒内化、融合和膜穿透方面的功能对于确定病毒的宿主范围限制和毒力至关重要。

Virus

HEK293

C-His

H8PF45 (M1-E340)

/

Approximately 38.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.