HAO1 蛋白, Human (Trx-His)

The HAO1 protein is a (S)-2-hydroxyacid oxidase with broad substrate specificity that facilitates glycolate oxidation. This activity produces glyoxylic acid, a key precursor for glycine synthesis. HAO1 Protein, Human (Trx-His) is the recombinant human-derived HAO1 protein, expressed by E. coli , with N-Trx, N-6*His labeled tag. The total length of HAO1 Protein, Human (Trx-His) is 370 a.a., with molecular weight of ~56.0 kDa.

HAO1 蛋白作为 (S)-2-羟基酸氧化酶表现出广泛的底物特异性，对乙醇酸氧化具有显着的偏好。这种酶活性产生乙醛酸，这是丙氨酸乙醛酸转氨酶在甘氨酸过氧化物酶体合成中所利用的重要前体。该途径在乙醛酸解毒、防止其积累方面发挥着至关重要的作用，否则可能导致肾结石的形成。此外，HAO1可以催化乙醛酸和长链羟基酸的氧化，例如2-羟基十六酸和2-羟基辛酸，但催化效率较低。虽然人工电子受体 2,6-二氯苯酚靛酚 (DCIP) 在体外具有活性，但据信 O2 充当生理电子受体，导致 H2O2 的产生。值得注意的是，HAO1 对 L-乳酸和 2-羟基丁酸不表现出活性。这种多方面的酶功能强调了其在多种代谢途径中的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-Trx;N-6*His

Q9UJM8 (M1-I370)

54363  [NCBI]

MLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI

Approximately 56.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.