1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. HARS/HisRS 蛋白, Human (sf9, His)

HARS/HisRS 蛋白, Human (sf9, His)

目录号: HY-P73933
产品使用指南

HARS 是一种关键酶,通过形成氨酰基腺苷酸中间体 (His-AMP),协调组氨酸与其相应 tRNA 3' 端的 ATP 依赖性连接。蛋白质合成中的这一关键步骤确保组氨酸准确掺入新生多肽链中。除了在翻译中的基本作用之外,HARS 还作为一个多功能参与者出现,为轴突引导过程做出贡献。 HARS 参与轴突引导凸显了它参与更广泛的细胞功能,使其重要性超出了其在氨酰化中的典型作用。HARS/HisRS 蛋白, Human (sf9, His) 是重组的 HARS/HisRS 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。HARS/HisRS 蛋白, Human (sf9, His) 全长 508 个氨基酸,分子量约为 ~59.6 kDa。

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描述

HARS, a pivotal enzyme, orchestrates the ATP-dependent ligation of histidine to the 3'-end of its corresponding tRNA by forming an aminoacyl-adenylate intermediate (His-AMP). This critical step in protein synthesis ensures the accurate incorporation of histidine into nascent polypeptide chains. Beyond its fundamental role in translation, HARS also emerges as a multifunctional player, contributing to axon guidance processes. The involvement of HARS in axon guidance highlights its participation in broader cellular functions, extending its significance beyond its canonical role in aminoacylation. HARS/HisRS Protein, Human (sf9, His) is the recombinant human-derived HARS/HisRS protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of HARS/HisRS Protein, Human (sf9, His) is 508 a.a., with molecular weight of ~59.6 kDa.

研究背景

HARS 是一种关键酶,通过形成氨酰基腺苷酸中间体 (His-AMP),协调组氨酸与其相应 tRNA 3' 端的 ATP 依赖性连接。蛋白质合成中的这一关键步骤确保组氨酸准确掺入新生多肽链中。除了在翻译中的基本作用之外,HARS 还作为一个多功能参与者出现,为轴突引导过程做出贡献。 HARS 参与轴突引导凸显了它参与更广泛的细胞功能,使其重要性超出了其在氨酰化中的典型作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

P12081 (A2-C509)

基因 ID

3035  [NCBI]

蛋白结构
N-term
His
HARS1 (A2-C509)
Accession # P12081
C-term
同用名
Histidine--tRNA ligase, cytoplasmic; HisRS; HARS1; HARS; HRS
分子量

Approximately 59.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HARS/HisRS Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HARS/HisRS Protein, Human (sf9, His)
目录号:
HY-P73933
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