HAS2 蛋白, Mouse (His)

The HAS2 protein adds GlcNAc or GlcUA monosaccharides to hyaluronic acid, playing a crucial role in its synthesis. HAS2 Protein, Mouse (His) is the recombinant mouse-derived HAS2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HAS2 Protein, Mouse (His) is 308 a.a., with molecular weight of ~39.9 kDa.

HAS2 蛋白通过催化 GlcNAc 或 GlcUA 单糖添加到新生透明质酸聚合物中，在透明质酸合成中发挥关键作用。作为参与该过程的关键同工酶，HAS2 对高分子透明质酸（大多数细胞外基质的主要成分）的形成有显着贡献。这种透明质酸聚合物在组织结构中发挥着结构作用，在调节细胞粘附、迁移和分化方面发挥着至关重要的作用。HAS2 的重要性延伸至发育过程，因为它是心内膜垫细胞转变为间充质细胞所必需的，这是心脏发育的关键步骤。此外，HAS2 可能在血管生成中发挥作用。HAS2 合成高分子透明质酸在早期接触抑制中尤其值得注意，早期接触抑制是细胞与其他细胞或细胞外基质接触时停止生长的细胞过程。总之，HAS2 在透明质酸介导的细胞和发育功能的复杂协调中发挥着核心作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

E. coli

N-6*His

P70312 (E67-L374)

15117  [NCBI]

EHRKMKKSLETPIKLNKTVALCIAAYQEDPDYLRKCLQSVKRLTYPGIKVVMVIDGNSDDDLYMMDIFSEVMGRDKSATYIWKNNFHEKGPGETEESHKESSQHVTQLVLSNKSICIMQKWGGKREVMYTAFRALGRSVDYVQVCDSDTMLDPASSVEMVKVLEEDPMVGGVGGDVQILNKYDSWISFLSSVRYWMAFNIERACQSYFGCVQCISGPLGMYRNSLLHEFVEDWYNQEFMGNQCSFGDDRHLTNRVLSLGYATKYTARSKCLTETPIEYLRWLNQQTRWSKSYFREWLYNAMWFHKHHL

Approximately 39.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.