2. 重组蛋白
  3. Viral Proteins
  4. HCoV-HKU1 Spike/S1 蛋白 (Q0ZME7, HEK293, His)

HCoV-HKU1 Spike/S1 蛋白 (Q0ZME7, HEK293, His)

目录号: HY-P77387
产品使用指南

HCoV-HKU1 刺突蛋白通过与宿主受体结合来启动病毒感染，促进病毒颗粒附着在细胞膜上。作为 I 类病毒融合蛋白，它通过一系列构象状态协调病毒粒子和细胞膜之间的融合。这些包括融合前天然状态、发夹前中间状态和融合后发夹状态。融合过程中涉及七肽重复的结构重排对于病毒和靶细胞膜的融合至关重要。HCoV-HKU1 Spike/S1 蛋白 (Q0ZME7, HEK293, His) 是重组的 HCoV-HKU1 刺突/S1 蛋白，由 HEK293 表达，带有 C-His 标签。HCoV-HKU1 Spike/S1 蛋白 (Q0ZME7, HEK293, His) 全长 744 个氨基酸，分子量约为 ~85.2 KDa。

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描述

HCoV-HKU1 Spike Proteinas initiates viral infection by binding to host receptors, facilitating virion attachment to the cell membrane. Operating as a class I viral fusion protein, it orchestrates fusion between the virion and cellular membranes through a series of conformational states. These include a pre-fusion native state, a pre-hairpin intermediate state, and a post-fusion hairpin state. The structural rearrangement during fusion, involving heptad repeats, is essential for merging viral and target cell membranes. HCoV-HKU1 Spike/S1 Protein (Q0ZME7, HEK293, His) is the recombinant Virus-derived HCoV-HKU1 Spike/S1 protein, expressed by HEK293, with C-His labeled tag. The total length of HCoV-HKU1 Spike/S1 Protein (Q0ZME7, HEK293, His) is 744 a.a., with molecular weight of ~85.2 KDa.
研究背景

HCoV-HKU1 Spike/S1 蛋白通过与宿主受体相互作用将病毒颗粒附着在细胞膜上，从而启动感染，从而在病毒感染过程中发挥关键作用。作为 I 类病毒融合蛋白，它介导病毒粒子与细胞膜的融合。该蛋白质经历至少三种构象状态：融合前天然状态、发夹前中间状态和融合后发夹状态。在病毒和靶细胞膜之间的融合过程中，卷曲螺旋区域（七肽重复序列）采用发夹三聚体结构，将融合肽定位在紧邻胞外域的C端区域的位置。这种结构重排对于驱动病毒和靶细胞膜的并置和随后的融合至关重要。
种属

Virus
表达系统

HEK293
标签

C-His
蛋白编号

Q0ZME7 (A13-R756)
基因 ID

/
蛋白结构
N-term
HCoV-HKU1 S1 (A13-R756)
Accession # Q0ZME7
His
C-term
同用名
Human coronAvirus HKU1 (isolate N5) (HCoV-HKU1) Spike/S1 Protein (S1, His)
分子量

Approximately 85.2 kDa.
纯度

Greater than 85% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HCoV-HKU1 Spike/S1 Protein (Q0ZME7, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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