2. 重组蛋白
  3. Viral Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin 蛋白, H3N2 (P03438, HEK293, His)

HA/Hemagglutinin 蛋白, H3N2 (P03438, HEK293, His)

目录号: HY-P75078
产品使用指南

HA/血凝素蛋白附着在细胞表面受体上，从而允许病毒附着。它决定宿主范围和毒力。作为 I 类病毒融合蛋白，它介导病毒和内体膜的融合以进行细胞渗透。在酸性内体中，HA2 发生不可逆的构象变化，释放融合肽。融合孔的形成需要多个HA三聚体。HA/Hemagglutinin 蛋白, H3N2 (P03438, HEK293, His) 是重组的 HA/血凝素蛋白，由 HEK293 表达，带有 C-His 标签。HA/Hemagglutinin 蛋白, H3N2 (P03438, HEK293, His) 全长 530 个氨基酸，分子量约为 ~71.3 kDa。

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描述

The HA/Hemagglutinin Protein attaches to cell surface receptors, allowing virus attachment. It determines host range and virulence. As a class I viral fusion protein, it mediates fusion of virus and endosomal membranes for cell penetration. In acidic endosomes, HA2 undergoes irreversible conformational change, releasing fusion peptide. Multiple HA trimers are needed for fusion pore formation. HA/Hemagglutinin Protein, H3N2 (P03438, HEK293, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by HEK293, with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H3N2 (P03438, HEK293, His) is 530 a.a., with molecular weight of ~71.3 kDa.
研究背景

HA/血凝素蛋白与细胞表面上含唾液酸的受体结合，促进病毒颗粒的附着。这种附着导致约三分之二的病毒颗粒通过网格蛋白依赖性内吞作用内化，约三分之一通过网格蛋白和小窝蛋白独立途径内化。它在确定宿主范围限制和毒力方面起着至关重要的作用。作为I类病毒融合蛋白，它负责通过介导内吞病毒颗粒膜与内体膜的融合来穿透细胞质。在内体的低pH环境下，HA2发生不可逆的构象变化，释放出融合疏水肽。HA 的多个三聚体对于形成有能力的融合孔是必需的。
种属

Virus
表达系统

HEK293
标签

C-His
蛋白编号

P03438 (M1-W530)
基因 ID

/
蛋白结构
N-term
H3N2 HA (M1-W530)
Accession # P03438
His
C-term
同用名
Influenza A H3N2 (A/X-31) Hemagglutinin / HA Protein (HEK293, His)
分子量

Approximately 71.3 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HA/Hemagglutinin Protein, H3N2 (P03438, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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