HA/Hemagglutinin 蛋白, H7N8 (ACR59554, sf9, His)

Avian influenza virus (AIV) belongs to the genus Orthomyxovirus and contains eight single-stranded negative-sense RNA segments (PB2, PB1, PA, HA, NP, NA, M, and NS). The haemagglutinin (HA) protein presents as a homotrimer in which each monomer consists of two di-sulfide-linked HA1 and HA2 subunits after the cleavage of the HA0 precursor. The HA protein, which is responsible for receptor binding and membrane fusion, plays a key role in the influenza virus entry pathway^[1][2]. HA/Hemagglutinin Protein, H7N8 (ACR59554, sf9, His) is the recombinant Virus-derived HA/Hemagglutinin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HA/Hemagglutinin Protein, H7N8 (ACR59554, sf9, His) is 535 a.a., with molecular weight of ~59 KDa.

禽流感 (AI) 病毒属于正粘病毒科、甲型流感病毒属，并含有负义八段 RNA 基因组(聚合酶基本 2，PB2；聚合酶基本 1，PB1；聚合酶酸，PA；血凝素，HA；核衣壳蛋白，NP，神经氨酸酶，NA；矩阵，M；非结构蛋白，NS)。LPAI病毒 H5 和 H7 亚型的 HA 裂解位点中碱性氨基酸的多次替换或插入使其能够被常见的细胞蛋白酶裂解，并且突变的病毒可以在宿主中全身传播，导致高致病性表型^[1]。
一些研究已证实，去除 HA 的 N-连接糖基化序列有助于增加 HA 与 α-2,6-连接唾液酸受体的亲和力，并减少病毒的生长和传播。不同 AIV 毒株的病毒脱落和传播差异取决于特定的 HA 和/或 NA 蛋白^[2]。

Virus

Sf9 insect cells

C-His

ACR59554 (M1-F535)

/

Approximately 59 kDa.

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.