1. 重组蛋白
  2. Cytokines and Growth Factors CAR-T related Proteins CD Antigens Receptor Proteins Enzymes & Regulators Biotinylated Proteins
  3. EGF Superfamily ErbB2/HER2 Stem Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. ErbB2/CD340 EGFR/ErbB family
  5. HER2/CD340 蛋白, Human (Biotinylated, HEK293, His-Avi)

HER2/CD340 蛋白, Human (Biotinylated, HEK293, His-Avi)

目录号: HY-P78138
COA 产品使用指南

HER2/CD340 蛋白是一种动态酪氨酸激酶,在神经调节蛋白受体复合物中至关重要,并调节微管动力学。激活后,它会触发 MEMO1-RHOA-DIAPH1 通路,抑制 GSK3B 并促进 APC 和 CLASP2 在细胞膜上的结合,从而实现微管稳定。HER2/CD340 蛋白, Human (Biotinylated, HEK293, His-Avi) 是重组的 HER2/CD340 蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。HER2/CD340 蛋白, Human (Biotinylated, HEK293, His-Avi) 全长 630 个氨基酸,分子量为 80-100 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μg ¥2450 In-stock
50 μg ¥5400 In-stock
100 μg ¥9180 询价
> 100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics. Upon activation, it triggers the MEMO1-RHOA-DIAPH1 pathway, inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization. HER2/CD340 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived HER2/CD340 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of HER2/CD340 Protein, Human (Biotinylated, HEK293, His-Avi) is 630 a.a., with molecular weight of 80-100 kDa.

研究背景

HER2/CD340 蛋白是一种动态蛋白酪氨酸激酶,是多种细胞表面受体复合物中的关键成分,需要辅助受体来实现有效的配体结合。至关重要的是,它作为神经调节蛋白受体复合物的一部分发挥着不可或缺的作用,GP30 被确定为该受体的潜在配体。除了其受体功能外,HER2/CD340 蛋白还复杂地调节外周微管 (MT) 的生长和稳定。激活后,由 ERBB2 激活启动的 MEMO1-RHOA-DIAPH1 信号通路会协调细胞膜上 GSK3B 的磷酸化和随后的抑制。这种策略性抑制可防止 APC 和 CLASP2 的磷酸化,促进它们与细胞膜的结合。值得注意的是,膜结合的 APC 能够将 MACF1 定位到细胞膜上,这是微管捕获和稳定的先决条件。在细胞核内,HER2/CD340 蛋白积极参与转录调控,与 PTGS2/COX-2 启动子中的 5'-TCAAATTC-3' 序列结合以激活转录。此外,它通过 RNA Pol I 参与 rRNA 基因的转录,增强了蛋白质合成,有助于整体细胞生长。HER2/CD340 蛋白的多方面活性强调了其在协调不同细胞过程(从受体信号传导到微管动力学和转录调节)中的核心作用。

生物活性

Measured by its binding ability in a functional ELISA. When immobilized Anti-Her2 Antibody,hFc Tag at 0.5 μg/ml (100 μl/Well), can bind Biotinvlated Human Her2,His Tag and the EC50 is <11 ng/mL.

种属

Human

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

P04626 (T23-T652)

基因 ID
蛋白结构
N-term
HER2 (T23-T652)
Accession # P04626
His-Avi
C-term
同用名
CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; NGL; p185erbB2; TKR1; ERBB2; HER-2/neu; NEU; ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV
分子量

80-100 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
HER2/CD340 Protein, Human (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78138
需求量: