1. 重组蛋白
  2. Cytokines and Growth Factors CAR-T related Proteins CD Antigens Receptor Proteins Enzymes & Regulators
  3. EGF Superfamily ErbB2/HER2 Stem Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. ErbB2/CD340 EGFR/ErbB family
  5. HER2/CD340 蛋白, Human (S310Y, HEK293, His)

HER2/CD340 蛋白, Human (S310Y, HEK293, His)

目录号: HY-P78680
COA 产品使用指南

HER2/CD340 蛋白是一种动态酪氨酸激酶,在神经调节蛋白受体复合物中至关重要,并调节微管动力学。激活后,它会触发 MEMO1-RHOA-DIAPH1 通路,抑制 GSK3B 并促进 APC 和 CLASP2 在细胞膜上的结合,以实现微管稳定。在细胞核中,HER2/CD340 蛋白激活 PTGS2/COX-2 和 rRNA 基因,有助于转录调节和蛋白质合成。其多方面的活动强调了其在细胞过程、跨越受体信号传导、微管动力学和转录控制中的核心作用。HER2/CD340 蛋白, Human (S310Y, HEK293, His) 是重组的 HER2/CD340 蛋白,由 HEK293 表达,带有 C-10*His 标签和 S310Y 突变。HER2/CD340 蛋白, Human (S310Y, HEK293, His) 全长 630 个氨基酸,分子量为 80-100 kDa。

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描述

HER2/CD340 Protein, a dynamic tyrosine kinase, is crucial in neuregulin-receptor complexes and regulates microtubule dynamics. Upon activation, it triggers the MEMO1-RHOA-DIAPH1 pathway, inhibiting GSK3B and facilitating APC and CLASP2 association at the cell membrane for microtubule stabilization. In the nucleus, HER2/CD340 Protein activates PTGS2/COX-2 and rRNA genes, contributing to transcriptional regulation and protein synthesis. Its multifaceted activities emphasize its central role in cellular processes, spanning receptor signaling, microtubule dynamics, and transcriptional control. HER2/CD340 Protein, Human (S310Y, HEK293, His) is the recombinant human-derived HER2/CD340 protein, expressed by HEK293 , with C-10*His labeled tag and S310Y mutation. The total length of HER2/CD340 Protein, Human (S310Y, HEK293, His) is 630 a.a., with molecular weight of 80-100 kDa.

研究背景

HER2/CD340 蛋白是一种动态蛋白酪氨酸激酶,是多种细胞表面受体复合物中的关键成分,需要辅助受体来实现有效的配体结合。至关重要的是,它作为神经调节蛋白受体复合物的一部分发挥着不可或缺的作用,GP30 被确定为该受体的潜在配体。除了其受体功能外,HER2/CD340 蛋白还复杂地调节外周微管 (MT) 的生长和稳定。激活后,由 ERBB2 激活启动的 MEMO1-RHOA-DIAPH1 信号通路会协调细胞膜上 GSK3B 的磷酸化和随后的抑制。这种策略性抑制可防止 APC 和 CLASP2 的磷酸化,促进它们与细胞膜的结合。值得注意的是,膜结合的 APC 能够将 MACF1 定位到细胞膜上,这是微管捕获和稳定的先决条件。在细胞核内,HER2/CD340 蛋白积极参与转录调节,与 PTGS2/COX-2 启动子中的 5'-TCAAATTC-3' 序列结合以激活转录。此外,它通过 RNA Pol I 参与 rRNA 基因的转录,增强了蛋白质合成,有助于整体细胞生长。 HER2/CD340 蛋白的多方面活性强调了其在协调多种细胞过程(从受体信号传导到微管动力学和转录调节)中的核心作用。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Hurman HER2 at 1 μ g/mL can bind Anti-HER2 antibody. The EC50 is 0.9928-1.44 8 ng/mL.

种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

P04626 (T23-T652, S310Y)

基因 ID
蛋白结构
N-term
HER2 (T23-T652, S310Y)
Accession # P04626
10*His
C-term
同用名
ERBB2; CD340; HER-2/neu; HER2; MLN19; NEU; NGL; TKR1
分子量

80-100 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized a 0.22 μm filtered solution of20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HER2/CD340 Protein, Human (S310Y, HEK293, His)
目录号:
HY-P78680
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