Her2/CD340 蛋白, Human (Biotinylated, HEK293, His)

HER2/CD340 Protein, a dynamic tyrosine kinase, is crucial in neuregulin-receptor complexes and regulates microtubule dynamics. Upon activation, it triggers the MEMO1-RHOA-DIAPH1 pathway, inhibiting GSK3B and facilitating APC and CLASP2 association at the cell membrane for microtubule stabilization. In the nucleus, HER2/CD340 Protein activates PTGS2/COX-2 and rRNA genes, contributing to transcriptional regulation and protein synthesis. Its multifaceted activities emphasize its central role in cellular processes, spanning receptor signaling, microtubule dynamics, and transcriptional control. Her2/CD340 Protein, Human (Biotinylated, HEK293, His) is the recombinant human-derived Her2/CD340 protein, expressed by HEK293, with C-His labeled tag. The total length of Her2/CD340 Protein, Human (Biotinylated, HEK293, His) is 630 a.a., with molecular weight of 80-110 kDa.

HER2/CD340 蛋白是一种动态蛋白酪氨酸激酶，是多种细胞表面受体复合物中的关键成分，需要辅助受体来实现有效的配体结合。至关重要的是，它作为神经调节蛋白受体复合物的一部分发挥着不可或缺的作用，GP30 被确定为该受体的潜在配体。除了其受体功能外，HER2/CD340 蛋白还复杂地调节外周微管 (MT) 的生长和稳定。激活后，由 ERBB2 激活启动的 MEMO1-RHOA-DIAPH1 信号通路会协调细胞膜上 GSK3B 的磷酸化和随后的抑制。这种策略性抑制可防止 APC 和 CLASP2 的磷酸化，促进它们与细胞膜的结合。值得注意的是，膜结合的 APC 能够将 MACF1 定位到细胞膜上，这是微管捕获和稳定的先决条件。在细胞核内，HER2/CD340 蛋白积极参与转录调节，与 PTGS2/COX-2 启动子中的 5'-TCAAATTC-3' 序列结合以激活转录。此外，它通过 RNA Pol I 参与 rRNA 基因的转录，增强了蛋白质合成，有助于整体细胞生长。HER2/CD340 蛋白的多方面活性强调了其在协调多种细胞过程（从受体信号传导到微管动力学和转录调节）中的核心作用。

1. Immobilized Anti-Her2 Antibody, hFc Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Biotinylated Human Her2, His Tag with the EC₅₀ of 10 ng/mL determined by ELISA.
2. Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED₅₀ this effect is 0.5829 μg/mL in the presence of 0.6 µg/mL Trastuzmab, corresponding to a specific activity is 1.72×10³ units/mg.

Human

HEK293

C-His

P04626 (T23-T652)

2064  [NCBI]

TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLT

80-110 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.