HEXA/Hexosaminidase A 蛋白, Human (sf9, His)

HEXA (hexosaminidase A) proteolytically hydrolyzes N-acetyl-D-hexosamine and/or sulfated residues in glycocomplexes, including oligosaccharides, glycolipids, and mucopolysaccharides. The activity of isoenzyme S towards anionic disulfated glycans is comparable to that of isoenzyme A, while isoenzyme B can effectively hydrolyze neutral oligosaccharides but cannot hydrolyze anionic disulfated substrates. HEXA/Hexosaminidase A Protein, Human (sf9, His) is the recombinant human-derived HEXA/Hexosaminidase A protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of HEXA/Hexosaminidase A Protein, Human (sf9, His) is 529 a.a., with molecular weight of ~64 kDa.

HEXA（己糖胺酶 A）蛋白在水解各种糖复合物中发现的非还原端 N-乙酰基-D-己糖胺和/或硫酸化 N-乙酰基-D-己糖胺残基方面发挥着关键作用，包括来自蛋白质、中性糖脂、和特定的粘多糖。同工酶 S 对阴离子双硫酸化聚糖（如 6-硫酸软骨素三糖 (C6S-3)、硫酸皮肤素五糖和硫酸化鞘糖脂 SM2）表现出与同工酶 A 相当的活性。相比之下，同工酶 B 可有效水解中性寡糖，但不作用于这些阴离子双硫酸化底物。重要的是，在 GM2A 存在的情况下，只有同工酶 A 负责降解 GM2 神经节苷脂，这显示了其在该生化过程中的特异性和核心作用。

Human

Sf9 insect cells

C-His

P06865 (M1-T529)

3073  [NCBI]

MTSSRLWFSLLLAAAFAGRATALWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPYLTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETFSQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFHWHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGDEVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSHFRCELLRRGVQAQPLNVGFCEQEFEQT

Approximately 64 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.