1. 重组蛋白
  2. Cytokines and Growth Factors
  3. HGF & Receptors
  4. HGF
  5. HGF 蛋白, Canine (HEK293)

HGF 蛋白, Canine (HEK293)

目录号: HY-P74889
产品使用指南

HGF 蛋白是一种有效的肝细胞有丝分裂原,可刺激成熟肝细胞增殖,并在不同组织中表现出多种生长因子特性。作为 MET 受体酪氨酸激酶的配体,HGF 在结合后启动下游信号级联,在 TMPRSS13 裂解后激活 MAPK 信号传导。HGF 蛋白, Canine (HEK293) 是重组的 HGF 蛋白,由 HEK293 表达,不带标签。HGF 蛋白, Canine (HEK293) 全长 699 个氨基酸,分子量约为 ~79.9 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

HGF protein is a potent hepatocyte mitogen that stimulates mature hepatocyte proliferation and exhibits multiple growth factor properties in different tissues. As a ligand of MET receptor tyrosine kinase, HGF initiates downstream signaling cascades upon binding, activating MAPK signaling after TMPRSS13 cleavage. HGF Protein, Canine (HEK293) is the recombinant canine-derived HGF protein, expressed by HEK293 , with tag free. The total length of HGF Protein, Canine (HEK293) is 699 a.a., with molecular weight of ~79.9 kDa.

研究背景

HGF 蛋白是一种有效的有丝分裂原,具有保肝特性,特别促进成熟肝实质细胞的增殖。除了以肝细胞为中心的功能外,HGF 还作为一种多功能生长因子,在广泛的组织和细胞类型中发挥作用。作为受体酪氨酸激酶 MET 的激活配体,HGF 与 MET 结合,促进受体二聚化并启动下游信号级联。此外,在 TMPRSS13 裂解和激活后,HGF 会激活 MAPK 信号传导,从而产生多种细胞效应。在结构上,HGF以二聚体形式存在,由通过二硫键连接的α链和β链组成。值得注意的是,它与 SRPX2 相互作用,增强 HGF 的促有丝分裂活性。这种多方面的功能强调了 HGF 在细胞生长、组织稳态和信号转导途径中的关键作用。

种属

Canine

表达系统

HEK293

标签

Tag Free

蛋白编号

Q867B7/NP_001002964.1 (Q32-S730)

基因 ID

403441  [NCBI]

蛋白结构
N-term
HGF (Q32-S730)
Accession # Q867B7
C-term
同用名
Hepatocyte growth factor; F-TCF; Hepatopoeitin-A; HGF; HGFB; HPTA; SF
分子量

Approximately 79.9 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HGF Protein, Canine (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HGF Protein, Canine (HEK293)
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HY-P74889
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