HGF 蛋白, Human (HEK293, His)

HGF Protein is a hepatocyte growth factor involved in signaling pathways including MET and MAPK. The HGF Protein consists of 728 amino acids, with an alpha chain containing an n-terminal domain four kringle domains, and a β serine protease domain. HGF Protein, Human (HEK293, His) is the recombinant human-derived HGF protein, expressed by HEK293 , with C-His labeled tag. The total length of HGF Protein, Human (HEK293, His) is 697 a.a., with molecular weight of 32-36 & 52 & 80-100 kDa, respectively.

Hepatocyte growth factor (HGF) 是肝细胞的有效丝裂原，作为广泛的组织和细胞类型的生长因子。HGF 是Met 受体酪氨酸激酶的激活配体，其活性对正常的细胞发育和器官再生至关重要，但Met 的异常激活与许多类型实体瘤的生长、侵袭和转移有关。HGF 在TMPRSS13 切割和激活后激活MAPK 信号。HGF 是一种由728 个氨基酸组成的双链蛋白，其α 链含有一个 N 端结构域和4 个kringle 结构域，β 有一个丝氨酸蛋白酶结构域。HGF 是纤溶酶原相关生长因子家族的一员，作为一种无活性的单链前体分泌，经过蛋白水解处理形成具有生物活性的二硫化物连接的α/β 异源二聚体。

1.The ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells has an ED₅₀ value of 0.3-1.6 ng/mL.
2.Immobilized Human HGF, His Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC₅₀ of ≤28.2 ng/mL determined by ELISA.

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  Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED₅₀ for this effect is 1.6 ng/ml, corresponding to a specific activity is 6.25×10⁵ U/mg.

Human

HEK293

C-His

P14210 (Q32-S728)

3082  [NCBI]

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

32-36&52&80-100 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 500 mM NaCl, pH 8.0 or PBS, 200mM L-arginine, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.