HGF 蛋白, Rat (GST)

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth, acting as a hepatotrophic factor for various tissues. It activates MAPK signaling through TMPRSS13 cleavage and activates MET receptor tyrosine kinase by promoting its dimerization. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Rat (GST) is the recombinant rat-derived HGF protein, expressed by E. coli , with N-GST labeled tag. The total length of HGF Protein, Rat (GST) is 252 a.a., with molecular weight of ~55.0 kDa.

HGF 蛋白是一种有效的有丝分裂原，可刺激成熟肝实质细胞的生长。它作为肝营养因子发挥作用，并作为各种组织和细胞类型的生长因子。HGF 作为受体酪氨酸激酶 MET 的激活配体，促进其二聚化。此外，它还通过 TMPRSS13 裂解和激活来激活 MAPK 信号传导。在结构上，HGF由通过二硫键连接的α链和β链组成。它还与 SRPX2 相互作用，增强 HGF 的有丝分裂活性。

Rat

E. coli

N-GST

P17945 (477T-728L)

24446  [NCBI]

TIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

Approximately 55.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.