1. 重组蛋白
  2. Cytokines and Growth Factors CAR-T related Proteins Receptor Proteins Enzymes & Regulators
  3. HGF & Receptors c-Met/HGFR Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. HGFR 蛋白, Human (sf9, His-GST)

HGFR 蛋白, Human (sf9, His-GST)

目录号: HY-P72938
COA 产品使用指南

HGFR 蛋白是一种受体酪氨酸激酶,通过与肝细胞生长因子/HGF 配体结合来转导信号。它调节增殖、散射、形态发生和细胞存活。HGFR 蛋白, Human (sf9, His-GST) 是重组的 HGFR 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。HGFR 蛋白, Human (sf9, His-GST) 全长 435 个氨基酸,分子量约为 ~68 kDa。

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描述

The HGFR protein is a receptor tyrosine kinase that transduces signals by binding to hepatocyte growth factor/HGF ligand. It regulates proliferation, scattering, morphogenesis, and cell survival. HGFR Protein, Human (sf9, His-GST) is the recombinant human-derived HGFR protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of HGFR Protein, Human (sf9, His-GST) is 435 a.a., with molecular weight of ~68 kDa.

研究背景

HGFR 蛋白是一种受体酪氨酸激酶,通过与肝细胞生长因子/HGF 配体结合,充当细胞外基质的信号转导器。它在调节多种生理过程中发挥着关键作用,包括增殖、散射、形态发生和细胞存活。配体在细胞表面结合后,HGFR 在其胞内结构域上发生自磷酸化,为下游信号分子创建对接位点。被配体激活后,它与 PI3 激酶亚基 PIK3R1、PLCG1、SRC、GRB2、STAT3 或适配器 GAB1 相互作用,导致多个信号级联的激活,包括 RAS-ERK、PI3 激酶-AKT 和 PLCgamma- PKC。RAS-ERK 激活与形态发生效应相关,而 PI3K/AKT 协调促生存效应。在胚胎发育中,HGFR 信号传导有助于原肠胚形成、神经元前体的发育和迁移、血管生成和肾脏形成。在骨骼肌发育过程中,它对于肌肉祖细胞的迁移和次级成肌细胞的增殖至关重要。在成人中,它参与伤口愈合、器官再生、组织重塑,并促进造血细胞的分化和增殖。此外,在微生物感染的情况下,HGFR充当InlB内单核细胞增生李斯特菌的受体,介导病原体进入细胞。

生物活性

1. The specific activity was determined to be >10 nmol/min/mg using MBP as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) at 10 μg/mL (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/mL.

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

P08581 (K956-S1390)

基因 ID
蛋白结构
N-term
His-GST
HGFR (K956-S1390)
Accession # P08581
C-term
同用名
Hepatocyte growth factor receptor; HGF receptor; SF receptor; MET
氨基酸序列

KKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGSCRQVQYPLTDMSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAEMRPSFSELVSRISAIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSEDNADDEVDTRPASFWETS

分子量

Approximately 68 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 3 mM DTT

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HGFR Protein, Human (sf9, His-GST)
目录号:
HY-P72938
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