HGPRT 蛋白, Human (His)

HGPRT proteins play a key role in purine metabolism, promoting the conversion of guanine to guanosine monophosphate and hypoxanthine to inosine monophosphate. Through its enzymatic activity, HGPRT transfers the 5-phosphate ribosyl group from 5-phosphate ribose pyrophosphate to purine, making a significant contribution to the purine salvage pathway. HGPRT Protein, Human (His) is the recombinant human-derived HGPRT protein, expressed by E. coli , with N-6*His labeled tag. The total length of HGPRT Protein, Human (His) is 218 a.a., with molecular weight of ~30.0 kDa.

HGPRT 蛋白或次黄嘌呤鸟嘌呤磷酸核糖基转移酶是一种关键酶，在通过嘌呤补救途径生成嘌呤核苷酸方面发挥着核心作用。HGPRT 催化鸟嘌呤转化为单磷酸鸟苷 (GMP) 以及次黄嘌呤转化为单磷酸肌苷 (IMP)。该酶活性涉及将 5-磷酸核糖基基团从 5-磷酸核糖基焦磷酸转移到嘌呤底物上。通过促进这些反应，HGPRT 确保在必需核苷酸的合成中有效利用回收的嘌呤，从而有助于 DNA 和 RNA 合成等细胞过程。以 HGPRT 为核心的嘌呤补救途径对于维持细胞内嘌呤核苷酸库和整体核苷酸稳态至关重要（参考：未提供）。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P00492 (M1-A218)

3251  [NCBI]

MATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, 50% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.