HIF-1 alpha 蛋白, Human (His)

HIF-1 alpha is a key protein that responds to low oxygen levels. HIF-1 alpha Protein, Human (His) is the recombinant human-derived HIF-1 alpha protein, expressed by E. coli , with N-His labeled tag. The total length of HIF-1 alpha Protein, Human (His) is 252 a.a., with molecular weight of ~31 kDa.

HIF-1 α 作为主要转录调节因子，协调对缺氧的适应性反应，激活 40 多个基因的转录，这些基因对于氧气输送、代谢适应和细胞对低氧水平的反应至关重要。其靶基因包括促红细胞生成素、葡萄糖转运蛋白、糖酵解酶、血管内皮生长因子和HILPDA。该蛋白在胚胎血管形成、肿瘤血管生成和缺血性疾病的病理生理学中发挥重要作用。HIF-1 α 与 ARNT 形成异二聚体，与靶基因启动子中的缺氧反应元件 (HRE) 结合。其激活需要 CREBBP 和 EP300 等共激活剂，并且其活性通过与 NCOA1、NCOA2 和 APEX1 的相互作用而增强。此外，HIF-1 α 参与缺氧期间神经元的轴突分布和线粒体运输，并且在感染 SARS-CoV-2 后，有助于诱导单核细胞糖酵解和促炎症状态。它诱导 ACE2 和细胞因子的表达，促进 SARS-CoV-2 复制和单核细胞炎症反应。

Human

E. coli

N-His

Q16665 (R575-N826)

3091  [NCBI]

RSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Approximately 31 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 1 mM EDTA, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.