Histone deacetylase 1/HDAC1 蛋白, Human (His-SUMO)

Studies have confirmed that the histone deacetylase 1 (HDAC1) protein is a key enzyme that deacetylates lysine residues on core histones (H2A, H2B, H3, H4). This process establishes an epigenetic repressive signature that affects transcription, cell cycle, and developmental events. Histone deacetylase 1/HDAC1 Protein, Human (His-SUMO) is the recombinant human-derived Histone deacetylase 1/HDAC1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Histone deacetylase 1/HDAC1 Protein, Human (His-SUMO) is 482 a.a., with molecular weight of ~71-74 kDa.

组蛋白脱乙酰酶 1 (HDAC1) 蛋白作为关键酶，催化位于核心组蛋白 N 末端区域的赖氨酸残基（包括 H2A、H2B、H3 和 H4）的脱乙酰化。这种脱乙酰化过程有助于表观遗传抑制标签的建立，并在转录调控、细胞周期进展和发育事件中发挥关键作用。HDAC1 在大型多蛋白复合物中发挥作用，是组蛋白脱乙酰酶 NuRD 复合物的组成部分，积极参与染色质重塑。除了组蛋白之外，HDAC1 对非组蛋白靶标也表现出脱乙酰酶活性，包括 NR1D2、RELA、SP1、SP3 和 TSHZ3。这种多功能酶通过脱乙酰化调节 SP 蛋白（SP1 和 SP3）的功能，并且它形成 BRG1-RB1-HDAC1 复合物的一部分，该复合物对静息神经元中 CREST 介导的转录进行负调节。此外，HDAC1 作为蛋白质去巴豆酰化酶，介导组蛋白去巴豆酰化，从而扩展其酶库。HDAC1 的多方面活性强调了其在表观遗传调控和多种细胞过程中的核心作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-SUMO;N-6*His

Q13547 (M1-A482)

3065  [NCBI]

MAQTQGTRRKVCYYYDGDVGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKANAEEMTKYHSDDYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVASAVKLNKQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNYPLRDGIDDESYEAIFKPVMSKVMEMFQPSAVVLQCGSDSLSGDRLGCFNLTIKGHAKCVEFVKSFNLPMLMLGGGGYTIRNVARCWTYETAVALDTEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA

71-74 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.2 μm filtered solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4 or 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.