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Histone H2A 蛋白, Xenopus laevis

目录号: HY-P72330
产品使用指南 技术支持

组蛋白 H2A 是一种完整的核小体成分,与 H2B、H3 和 H4 形成组蛋白八聚体。该分子线轴由两个 H2A-H2B 异二聚体和一个 H3-H4 异四聚体组成,包裹着大约 147 个碱基对的 DNA,组织染色质结构。复杂的组蛋白-DNA 关联,尤其是与 H2A 的关联,在调节基因表达和 DNA 包装等细胞过程中发挥着至关重要的作用。Histone H2A 蛋白, Xenopus laevis 是重组的 组蛋白 H2A 蛋白,由 E. coli 表达,不带标签。

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规格 价格 是否有货 数量
10 μg ¥1950 1 - 2 周
50 μg ¥5500 1 - 2 周
100 μg   询价  

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描述

Histone H2A, an integral nucleosome component, forms the histone octamer with H2B, H3, and H4. This molecular spool, consisting of two H2A-H2B heterodimers and one H3-H4 heterotetramer, wraps around approximately 147 base pairs of DNA, organizing chromatin structure. The intricate histone-DNA association, especially with H2A, plays a vital role in regulating cellular processes like gene expression and DNA packaging. Histone H2A Protein, Xenopus laevis is the recombinant Xenopus laevis-derived Histone H2A protein, expressed by E. coli , with tag free.

研究背景

组蛋白 H2A 是核小体的重要组成部分,核小体是染色质组织的基本结构单位。核小体包含由 H2A、H2B、H3 和 H4 各两个拷贝组成的组蛋白八聚体,其中一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体。这种八聚体组装体充当分子线轴,大约 147 个 DNA 碱基对错综复杂地缠绕在该分子线轴周围,从而形成了紧凑且有组织的染色质结构。组蛋白(包括 H2A)与核小体中 DNA 的复杂关联对于调节各种细胞过程(包括基因表达和 DNA 包装)至关重要。

种属

Xenopus laevis

表达系统

E. coli

标签

Tag Free

蛋白编号

Q6AZJ8 (T17-L197)

基因 ID

494591  [NCBI]

蛋白结构
N-term
Histone H2A (T17-L197)
Accession # Q6AZJ8
C-term
蛋白长度

Partial

中文名
Histone H2A 蛋白
同用名
h2ac14.L
氨基酸序列

TRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAVRNDEELNKL

分子量

Approximately 12.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of ddH2O, pH 7.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Histone H2A Protein, Xenopus laevis 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Histone H2A Protein, Xenopus laevis
目录号:
HY-P72330
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