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Histone-lysine N-methyltransferase SMYD3(SMYD3) 蛋白, Human (His-Myc)

目录号: HY-P700258
产品使用指南

SMYD3 蛋白是一种组蛋白甲基转移酶,特异性诱导 H3K4 的二甲基化和三甲基化,避免单甲基化,并使 H4K5 甲基化。SMYD3 对于 RNA 聚合酶复合物内的转录激活至关重要,它还对包含 5'-CCCTCC-3' 或 5'-GAGGGG-3' 的序列表现出 DNA 结合亲和力。Histone-lysine N-methyltransferase SMYD3 (SMYD3) 蛋白, Human (His-Myc) 是重组的 组蛋白赖氨酸 N-甲基转移酶 SMYD3 蛋白,由 E. coli 表达,带有 N-10*His, C-Myc 标签。Histone-lysine N-methyltransferase SMYD3 (SMYD3) 蛋白, Human (His-Myc) ,分子量为 56.5 kDa。

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描述

SMYD3 Protein, a histone methyltransferase, specifically induces di- and tri-methylation of H3K4, avoiding monomethylation, and methylates H4K5. Crucial in transcriptional activation within an RNA polymerase complex, SMYD3 also exhibits DNA-binding affinity for sequences containing 5'-CCCTCC-3' or 5'-GAGGGG-3'. Histone-lysine N-methyltransferase SMYD3 (SMYD3) Protein, Human (His-Myc) is the recombinant human-derived Histone-lysine N-methyltransferase SMYD3(SMYD3) protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. Histone-lysine N-methyltransferase SMYD3 (SMYD3) Protein, Human (His-Myc), has molecular weight of 56.5 kDa.

研究背景

SMYD3 蛋白是一种组蛋白甲基转移酶,在甲基化组蛋白 H3 的“Lys-4”方面表现出特异性,诱导二甲基化和三甲基化,但不诱导单甲基化。此外,它还能甲基化组蛋白 H4 的“Lys-5”。在 RNA 聚合酶复合物中,SMYD3 在转录激活中发挥着至关重要的作用。该蛋白质因其能够结合含有 5'-CCCTCC-3' 或 5'-GAGGGG-3' 的 DNA 序列而闻名。

种属

Human

表达系统

E. coli

标签

N-10*His;C-Myc

蛋白编号
基因 ID
蛋白结构
N-term
10*His
SMYD3
Accession # Q9H7B4
C-term
氨基酸序列

MEPLKVEKFATAKRGNGLRAVTPLRPGELLFRSDPLAYTVCKGSRGVVCDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKRECKCLKSCKPRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHWKWEQVLAMCQAIISSNSERLPDINIYQLKVLDCAMDACINLGLLEEALFYGTRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS

分子量

56.5 kDa

纯度

Greater than 90% as determined by SDS-PAGE.

文件资料

Histone-lysine N-methyltransferase SMYD3(SMYD3) Protein, Human (His-Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Histone-lysine N-methyltransferase SMYD3(SMYD3) Protein, Human (His-Myc)
目录号:
HY-P700258
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