HLA-A 蛋白, Human (His)

HLA-A protein is an antigen-presenting MHCI molecule that is critical for mediating reproductive and antiviral immunity. It partners with B2M to provide self- and viral peptides as ligands that inhibit and activate KIR on NK cells. HLA-A Protein, Human (His) is the recombinant human-derived HLA-A protein, expressed by E. coli , with N-6*His labeled tag. The total length of HLA-A Protein, Human (His) is 284 a.a., with molecular weight of ~36.7 kDa.

HLA-C*0304 是一种抗原呈递主要组织相容性复合物 I 类 (MHCI) 分子，在生殖和抗病毒免疫中发挥着至关重要的作用。它与 B2M/beta 2 微球蛋白合作，表现出有限的自身肽和病毒肽，作为自然杀伤 (NK) 细胞上表达的抑制性和激活性杀伤免疫球蛋白受体 (KIR) 的重要配体。特别是在妊娠期间，HLA-C*0304 通过 KIR 介导绒毛外滋养细胞和子宫 NK 细胞之间的相互作用，从而调节对胎盘形成和胎儿整体生长至关重要的滋养细胞侵袭。在病毒感染期间，它呈现对 KIR 亲和力低的病毒肽，防止 KIR 介导的抑制并促进受感染细胞的裂解。此外，HLA-C*0304在抗原呈递细胞上展示有限的病毒肽库，促进CD8阳性T细胞上的α-βT细胞受体的识别，从而引导抗原特异性T细胞免疫反应以消除感染细胞，尤其是在慢性病毒感染情况下，例如 HIV-1 或 CMV 感染。肽和 MHC 分子共同促进抗原识别，肽决定精细特异性，MHC 残基决定 T 细胞受体限制。HLA-C*0304 通常呈递源自蛋白酶体胞质蛋白水解的 9 个氨基酸的胞内肽抗原，对 2 和 9 位具有特定锚定残基的肽表现出结合偏好，主要包含疏水性或芳香族氨基酸（Phe、Ile、Leu） 、Met、Val 和 Tyr）。

Human

E. coli

N-6*His

P30443 (G25-I308)

3105  [NCBI]

GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQPTIPI

Approximately 36.7 kDa

• 
  Greater than 91% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.