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  3. HMGB1/HMG-1 蛋白, Human (102a.a, His)

HMGB1/HMG-1 蛋白, Human (102a.a, His)

目录号: HY-P73103
COA 产品使用指南

多功能且氧化还原敏感的 HMGB1/HMG-1 蛋白在细胞区室中发挥多种作用。在细胞核中,它是一种主要的染色质相关非组蛋白,参与复制、转录、染色质重塑、V(D)J 重组、DNA 修复和基因组稳定性等关键过程。HMGB1/HMG-1 蛋白, Human (102a.a, His) 是重组的 HMGB1/HMG-1 蛋白,由 E. coli 表达,带有 N-His, N-6*His 标签。HMGB1/HMG-1 蛋白, Human (102a.a, His) 全长 102 个氨基酸,分子量约为 ~15 kDa。

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描述

The multifunctional and redox-sensitive HMGB1/HMG-1 protein plays multiple roles in cellular compartments. In the nucleus, it serves as a major chromatin-associated non-histone protein involved in key processes such as replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and genome stability. HMGB1/HMG-1 Protein, Human (102a.a, His) is the recombinant human-derived HMGB1/HMG-1 protein, expressed by E. coli , with N-His, N-6*His labeled tag. The total length of HMGB1/HMG-1 Protein, Human (102a.a, His) is 102 a.a., with molecular weight of ~15 kDa.

研究背景

HMGB1/HMG-1 是一种多功能氧化还原敏感蛋白,在不同的细胞区室中表现出不同的作用。在细胞核内,它是一种主要的染色质相关非组蛋白,作为 DNA 伴侣参与复制、转录、染色质重塑、V(D)J 重组、DNA 修复和基因组稳定性等关键过程。HMGB1/HMG-1 被提议作为核酸的通用生物传感器,在促进宿主对无菌和感染信号的炎症反应、协调先天性和适应性免疫反应方面发挥着关键作用。在细胞质中,它充当免疫原性核酸的传感器和/或伴侣,激活 TLR9 介导的免疫反应并介导自噬。HMGB1/HMG-1 释放到细胞外环境,与 DNA、核小体、IL-1 beta、CXCL12、AGER 异构体 2/sRAGE、脂多糖 (LPS) 和脂磷壁酸 (LTA) 等多种分子结合,通过多种方式激活细胞表面受体。细胞外HMGB1以不同的氧化还原状态存在,完全还原的HMGB1作为趋化因子,二硫键HMGB1作为细胞因子,磺酰基HMGB1促进免疫耐受。除了其免疫调节作用之外,HMGB1/HMG-1 还涉及促血管生成活性、血小板活化、通过 RAGE 的神经元生长信号传导,以及可能参与扩展的聚谷氨酰胺蛋白的积累。其核功能归因于完全减少的 HMGB1,包括与染色质关联、DNA 弯曲以及通过成环增强 DNA 灵活性,促进各种基因启动子活性。此外,HMGB1/HMG-1 可能在核苷酸切除修复、错配修复、碱基切除修复、双链断裂修复、V(D)J 重组、组蛋白 H1 置换、重组核小体、增强转录因子结合和调节中发挥作用。端粒酶复合物。HMGB1/HMG-1 的复杂功能凸显了其在多种细胞过程中的重要性。

生物活性

Measured by its ability to induce TNF-α secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.3708 μg/mL, corresponding to a specific activity is 2.7×103 U/mg.

  • Measured by its ability to induce TNF-α secretion by RAW 264.7 mouse monocyte/macrophage cells.The ED50 for this effect is 0.3708 μg/mL, corresponding to a specific activity is 2.7×103 U/mg.
种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P09429 (K57-D158)

基因 ID
蛋白结构
N-term
6*His
HMGB1 (K57-D158)
Accession # P09429
C-term
同用名
High mobility group protein B1; HMG-1; HMGB1; HMG1
氨基酸序列

KGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKD

分子量

Approximately 15 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

HMGB1/HMG-1 Protein, Human (102a.a, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HMGB1/HMG-1 Protein, Human (102a.a, His)
目录号:
HY-P73103
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