HMGCR 蛋白, Human (His)

As a key enzyme in cholesterol biosynthesis, HMGCR protein plays a central role in regulating cellular cholesterol levels. It catalyzes the conversion of HMG-CoA to mevalonate, a key step in the synthesis of cholesterol and other isoprenoids. HMGCR Protein, Human (His) is the recombinant human-derived HMGCR protein, expressed by E. coli , with N-6*His labeled tag. The total length of HMGCR Protein, Human (His) is 300 a.a., with molecular weight of ~38 kDa.

亲环蛋白 A 蛋白充当寡肽内脯氨酸酰亚胺肽键顺反异构化的催化剂。除了其酶促作用之外，它还发挥多种细胞作用，包括对白细胞的有效趋化影响，部分通过激活其膜受体 BSG/CD147 介导，启动导致 MAPK/ERK 激活的信号级联反应。该蛋白还以促炎方式激活内皮细胞 (EC)，诱导 NF-κ-B 和 MAP 激酶通路并促进粘附分子的表达。此外，Cyclophilin A 通过调节参与趋化和细胞凋亡的关键因子的表达来诱导 EC 细胞凋亡。为了应对氧化应激，它在 EC 中启动促凋亡和抗凋亡信号传导，凸显了其多方面的作用。该蛋白负向调节 MAP3K5/ASK1 激酶活性，对于异质核核糖核蛋白复合物中 TARDBP 的组装至关重要，影响 TARDBP 与 RNA 的结合并调节相关基因的表达。此外，亲环蛋白 A 在血小板活化和聚集以及通过增加 ROS 产生和促进整合素-细胞骨架相互作用来调节钙动员和整合素 ITGA2B:ITGB3 双向信号传导中发挥着重要作用。它还表现出对硫酸乙酰肝素糖胺聚糖的结合亲和力。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P04035 (M588-T887)

3156  [NCBI]

MTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKT

Approximately 38 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.