HNRNPM 蛋白, Human (His-SUMO)

The HNRNPM protein is a key pre-mRNA binding protein that exhibits strong affinity for poly(G) and poly(U) RNA homopolymers. It is central to the splicing process, complexly regulates RNA maturation, and serves as a receptor for carcinoembryonic antigen in Kupffer cells. HNRNPM Protein, Human (His-SUMO) is the recombinant human-derived HNRNPM protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HNRNPM Protein, Human (His-SUMO) is 729 a.a., with molecular weight of ~93.4 kDa.

HNRNPM 蛋白在体内成为重要的前 mRNA 结合蛋白，在体外对聚 (G) 和聚 (U) RNA 均聚物表现出很强的亲和力。HNRNPM 在功能上与剪接过程有关，在 RNA 成熟的复杂调节中发挥着重要作用。除了参与剪接之外，它还充当库普弗细胞中癌胚抗原的受体，可能启动一系列信号传导事件，导致蛋白质酪氨酸磷酸化和诱导细胞因子，例如 IL-1 α、IL-6、IL- 10、肿瘤坏死因子α。值得注意的是，HNRNPM 被确定为剪接体 C 复合物的一个组成部分，强调了它与基本 RNA 加工机制的关联。此外，该蛋白质与 PPIA/CYPA 相互作用，进一步扩展其分子相互作用网络并突出其在细胞过程中的多方面作用。

Human

E. coli

N-6*His;N-SUMO

P52272 (A2-A730)

4670  [NCBI]

AAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

Approximately 93.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.