2. 重组蛋白
  3. Enzymes & Regulators
  4. Isomerases (EC 5)
  5. HP-0175 蛋白, Helicobacter pylori (GST)

HP-0175 蛋白, Helicobacter pylori (GST)

目录号: HY-P700388
产品使用指南

HP-0175 是幽门螺杆菌分泌的一种抗原。HP-0175 通过促进促炎低细胞毒性 TIL 反应、基质降解和促血管生成途径，在幽门螺杆菌相关炎症和胃癌之间提供了联系。HP-0175 依赖于未折叠蛋白反应 (UPR) 在胃上皮细胞中进行自噬，诱导细胞凋亡。HP-0175 蛋白, Helicobacter pylori (GST) 是重组的 HP-0175 蛋白，由 E. coli 表达，带有 N-GST 标签。HP-0175 蛋白, Helicobacter pylori (GST) 全长 278 个氨基酸，分子量为 58.8 kDa。

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描述

HP-0175 is an antigen secreted by Helicobacter pylori. HP-0175 provides a link between helicobacter pylori-associated inflammation and gastric cancer by promoting the pro-inflammatory low-cytotoxic TIL response, stromal degradation, and pro-angiogenesis pathways. HP-0175 relies on the unfolded protein response (UPR) to autophagy in gastric epithelial cells and induce apoptosis. HP-0175 Protein, Helicobacter pylori (GST) is the recombinant HP-0175 protein, expressed by E. coli , with N-GST labeled tag. The total length of HP-0175 Protein, Helicobacter pylori (GST) is 278 a.a., with molecular weight of 58.8 kDa.
研究背景

HP-0175 是幽门螺杆菌分泌的一种抗原。HP-0175 是一种肽基脯氨酸顺式反式异构酶，对宿主细胞具有兼职作用。HP-0175 通过促进促炎低细胞毒性 TIL 反应、基质降解和促血管生成途径，可能在幽门螺杆菌相关炎症和胃癌之间提供了联系。HP-0175 依赖于未折叠蛋白反应 (UPR) 在胃上皮细胞中进行自噬，诱导细胞凋亡。
种属

Others
表达系统

E. coli
标签

N-GST
蛋白编号

P56112 (K22-K299)
基因 ID

/
蛋白结构
N-term
GST
HP-0175 (K22-K299)
Accession # P56112
C-term
同用名
Rotamase HP_0175; Putative peptidyl-prolyl cis-trans isomerase HP_0175
氨基酸序列

KPAHNANNATHNTKKTTDSSAGVLATVDGRPITKSDFDMIKQRNPNFDFDKLKEKEKEALIDQAIRTALVENEAKTEKLDSTPEFKAMMEAVKKQALVEFWAKKQAEEVKKVQIPEKEMQDFYNANKDQLFVKQEAHARHILVKTEDEAKRIISEIDKQPKAKKEAKFIELANRDTIDPNSKNAQNGGDLGKFQKNQMAPDFSKAAFALTPGDYTKTPVKTEFGYHIIYLISKDSPVTYTYEQAKPTIKGMLQEKLFQERMNQRIEELRKHAKIVINK

分子量

58.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

HP-0175 Protein, Helicobacter pylori (GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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