HSD11B1 蛋白, Human (His-SUMO)

The proteins encoded by ED genes are involved in a variety of physiological processes. HSD11B1 Protein, Human (His-SUMO) is the recombinant human-derived HSD11B1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HSD11B1 Protein, Human (His-SUMO) is 268 a.a., with molecular weight of ~45.5 kDa.

在 NADP(H) 存在的情况下，HSD11B1 蛋白控制生物活性糖皮质激素的可逆转化，例如可的松到皮质醇，以及 11-脱氢皮质酮到皮质酮。这种酶活性对于皮质类固醇受体介导的抗炎反应以及代谢和稳态过程至关重要。此外，HSD11B1 在眼房水的分泌中发挥作用，有助于维持正常血压的眼内环境。虽然在体外显示出双向能力，但它在体内主要充当还原酶，从而增加活性糖皮质激素的浓度。该酶表现出广泛的底物特异性，接受各种类固醇和甾醇底物。值得注意的是，它可以相互转化 7-氧代-和 7-羟基-神经类固醇，例如 7-氧代孕烯醇酮和 7β-羟基孕烯醇酮、7-氧代去氢表雄酮和 7β-羟基脱氢表雄酮等。此外，HSD11B1 催化主要膳食氧甾醇 7-酮胆固醇立体特异性转化为极性更强的 7-β-羟基胆固醇代谢物，这一过程对细胞凋亡、动脉粥样硬化病变、脂质过氧化和泡沫细胞形成有影响。此外，它介导 7-氧代石胆酸 7-氧代还原，在糖皮质激素激活和胆汁酸代谢之间建立联系，最终通过合成 7-β-25-二羟基胆固醇（G 蛋白的配体）影响免疫细胞迁移。偶联受体 Epstein-Barr 病毒诱导基因 2 (EBI2)。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P28845 (E25-K292)

3290  [NCBI]

EEFRPEMLQGKKVIVTGASKGIGREMAYHLAKMGAHVVVTARSKETLQKVVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHITNTSLNLFHDDIHHVRKSMEVNFLSYVVLTVAALPMLKQSNGSIVVVSSLAGKVAYPMVAAYSASKFALDGFFSSIRKEYSVSRVNVSITLCVLGLIDTETAMKAVSGIVHMQAAPKEECALEIIKGGALRQEEVYYDSSLWTTLLIRNPCRKILEFLYSTSYNMDRFINK

Approximately 45.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.