HSP60 蛋白, Human (His-GST)

The HSP60 protein is a mitochondrial chaperone that facilitates protein import and assembly. HSP60 Protein, Human (His-GST) is the recombinant human-derived HSP60 protein, expressed by E. coli , with N-His, N-GST labeled tag.

HSP60 蛋白作为伴侣蛋白，在线粒体蛋白输入和大分子组装中发挥关键作用。它与 Hsp10 合作，促进输入蛋白质的准确折叠，并且在线粒体基质的应激条件下，可以防止错误折叠，同时促进未折叠多肽的重折叠和正确组装。这些伴侣蛋白的结构单元包含大亚基Hsp60的七聚环，形成背靠背的双环。在循环过程中，Hsp60 环复合物每个环依次结合一个未折叠的底物蛋白，然后与辅助伴侣蛋白 Hsp10 的两个七聚环结合并缔合 ATP。这种循环相互作用导致底物蛋白被隔离在 Hsp60 的内腔内，使其能够在一定时间内不受其他细胞成分干扰地进行折叠。所有 Hsp60 亚基中 ATP 的同步水解导致伴侣蛋白环解离，从而释放 ADP 和正确折叠的底物蛋白（可能）。

Measured in a cell proliferation assay using A549 cells.The ED₅₀ for this effect is 0.213 μg/mL, corresponding to a specific activity is 4701.457 Unit/mg.

Human

E. coli

N-His;N-GST

P10809-1 (L2-F573)

3329  [NCBI]

Partial

LRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSIQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLTPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMAGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

52-88 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.