HSP90AA1 蛋白, Human (His-SUMO)

The HSP90AA1 protein is an important molecular chaperone that coordinates the maturation and regulation of specific target proteins critical for cell cycle control and signal transduction. Its ATPase activity drives a functional cycle that induces conformational changes in client proteins for activation. HSP90AA1 Protein, Human (His-SUMO) is the recombinant human-derived HSP90AA1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of HSP90AA1 Protein, Human (His-SUMO) is 224 a.a., with molecular weight of ~43 kDa.

HSP90AA1 蛋白是一种分子伴侣，在参与细胞周期控制和信号转导的特定靶蛋白的成熟、结构维持和调节中发挥着至关重要的作用。HSP90AA1 通过与其伴侣功能至关重要的 ATP 酶活性相关的功能循环进行操作，诱导客户蛋白的构象变化，从而激活它们。与共伴侣的动态相互作用调节底物识别、ATP 酶循环和伴侣功能。它通过辅助伴侣与不同的客户蛋白类别结合，形成功能性伴侣，以 ADP 结合的部分开放构象释放正确折叠的客户蛋白和辅助伴侣。除了其伴侣活性外，HSP90AA1 通过将前蛋白递送至线粒体输入受体 TOMM70，在线粒体输入中发挥着关键作用。它还有助于在多个水平上调节转录机制，改变转录因子水平，调节表观遗传修饰剂，并参与基因启动子中的组蛋白驱逐。此外，HSP90AA1 结合细菌脂多糖，介导 LPS 诱导的炎症反应，包括 TNF 分泌。它拮抗 STUB1 介导的 TGF-β 信号传导抑制，并促进 TOMM70 与线粒体外膜中的 IRF3 或 TBK1 结合，从而促进宿主抗病毒反应。在微生物感染的情况下，HSP90AA1 干扰脑膜炎奈瑟氏球菌 NadA 介导的对人类细胞的侵袭，根据其表达水平影响表达 NadA 的大肠杆菌粘附和进入人类 Chang 细胞。

Data is not available.

Human

E. coli

N-6*His;N-SUMO

P07900 (D9-D232)

3320  [NCBI]

DQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLIPNKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVTVITKHNDDEQYAWESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKHSQFIGYPITLFVEKERDKEVSD

Approximately 43 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0l or 50 mM Tris-HCL, 300 mM NaCL, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.