HSPA5/GRP-78 蛋白, Mouse (P.pastoris, His)

The HSPA5/GRP-78 protein is an endoplasmic reticulum chaperone involved in protein folding and quality control. It interacts with DNAJC10/ERdj5 to fold and degrade misfolded proteins. HSPA5/GRP-78 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived HSPA5/GRP-78 protein, expressed by P. pastoris , with N-6*His, N-His labeled tag. The total length of HSPA5/GRP-78 Protein, Mouse (P.pastoris, His) is 636 a.a., with molecular weight of ~72.5 kDa.

HSPA5/GRP-78 蛋白作为重要的内质网伴侣，在内质网腔内的蛋白质折叠和质量控制中发挥着关键作用。它参与正确的蛋白质折叠并参与错误折叠蛋白质的降解，与 DNAJC10/ERdj5 合作促进 DNAJC10/ERdj5 从其底物中释放。此外，HSPA5/GRP-78 是 ERN1/IRE1 介导的未折叠蛋白反应 (UPR) 的关键抑制因子。在无应激的内质网中，它被 DNAJB9/ERdj4 招募到 ERN1/IRE1 的管腔区域，破坏 ERN1/IRE1 的二聚化，从而使其失活。错误折叠蛋白的积累会触发 ERN1/IRE1 释放 HSPA5/BiP，从而实现同二聚化并随后激活 ERN1/IRE1。此外，HSPA5/GRP-78 在小前分泌蛋白跨内质网的翻译后转运中发挥辅助作用，并且可能充当 SEC61 通道形成易位子复合物的变构调节剂。建议与 SEC62 合作，将这些前体高效地插入 SEC61 通道中。该蛋白质似乎特异性调节成熟区域中带有抑制残基的前体的易位，从而削弱了通道门控。除了在蛋白质折叠中的作用之外，HSPA5/GRP-78 还可能有助于细胞凋亡和细胞增殖。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

P. pastoris

N-6*His

P20029 (20E-655L)

14828  [NCBI]

EEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL

Approximately 72.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCI, 0.5 M NaCI, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.