HSPA8/HSC70 蛋白, Human (N-His)

HSPA8/HSC70 is a molecular chaperone that protects the proteome and aids in peptide folding, transport, chaperone-mediated autophagy, and protein complex regulation. It is central to quality control, ensuring correct folding and targeting misfolded proteins for degradation through ATP-dependent cycles. HSPA8/HSC70 Protein, Human (N-His) is the recombinant human-derived HSPA8/HSC70 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HSPA8/HSC70 Protein, Human (N-His) is 646 a.a., with molecular weight of ~65 kDa.

HSPA8/HSC70 是一种分子伴侣，错综复杂地参与多种细胞过程，包括蛋白质组免受应激、促进多肽折叠和运输、伴侣介导的自噬、错误折叠蛋白水解的激活以及蛋白复合物形成和解离的调节。它是蛋白质质量控制系统的核心，确保正确的蛋白质折叠、错误折叠蛋白质的重折叠，并调节蛋白质靶向以进行后续降解。该功能是通过 ATP 结合、ATP 水解和 ADP 释放的循环来协调的，并由共伴侣促进。HSP70 的核苷酸结合状态调节其对多肽的亲和力，其中 ATP 结合形式具有低底物亲和力，而 ATP 水解时的构象变化增加了对底物的亲和力。辅助伴侣，包括 J 结构域辅助伴侣 (HSP40)、核苷酸交换因子 (NEF)（例如 BAG1/2/3）和 TPR 结构域伴侣（例如 HOPX 和 STUB1），在调节 HSP70 活性中发挥特定作用。除了在线粒体输入中的基本作用之外，HSPA8/HSC70 还充当转录激活的阻遏物，参与剪接体组装，并在选择性蛋白质降解过程中发挥作用，包括分子伴侣介导的自噬和 ER 相关降解。此外，它与 VGF 衍生肽 TLQP-21 相互作用，表明它参与多种细胞途径。

HSC70 has ATPase activity at the time of manufacture of 13.173 µM phosphate liberated/h/µg protein in a 200µL reaction at 37°C in the presence of 10 µL of 4 mM ATP using a Malachite Green assay.

Human

E. coli

N-6*His

P11142-1 (M1-D646)

3312

MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD

Approximately 65 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.