1. 重组蛋白
  2. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  3. TNF Superfamily Inhibitory Checkpoint Molecules Stimulatory Immune Checkpoint Molecules B Cell CD Proteins Macrophage CD Proteins Epithelial cell CD Proteins
  4. TNF Receptor Superfamily HVEM/CD270
  5. HVEM
  6. HVEM/TNFRSF14 蛋白, Human (164a.a, HEK293, His)

HVEM/TNFRSF14 蛋白, Human (164a.a, HEK293, His)

目录号: HY-P70489
COA 产品使用指南

HVEM (疱疹病毒入侵介质蛋白,TNFRSF14,CD270) 是肿瘤坏死因子受体超家族 (TNFRSF)的成员。 HVEM 是一种双向分子开关,可转换正信号和负信号。当与 BTLA 或 LIGHT 结合时,HVEM 可以传递促炎和存活信号,刺激淋巴细胞增殖、激活并诱导炎症反应。同时,HVEM 与 CD160 和 BTLA 结合,可抑制 T 和 B 淋巴细胞的活化和增殖。HVEM/TNFRSF14 蛋白, Human (164a.a, HEK293, His) 是一种由 HEK293 细胞表达的重组蛋白,其 C 末端带有 Fc 标签,由 164 个 (L39-V202) 氨基酸组成。

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10 μg ¥600 In-stock
50 μg ¥1800 In-stock
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描述

HVEM (herpes virus entry mediator, TNFRSF14, CD270) is a member of the tumor necrosis factor receptor superfamily (TNFRSF). HVEM is a bidirectional molecular switch that transduces positive and negative signals. HVEM can deliver proinflammatory and survival signals when engaged by BTLA or LIGHT, stimulating lymphocyte proliferation, activation, and inducing inflammatory reactions. While, HVEM binds to CD160 and BTLA, inhibiting T- and B-lymphocyte activation and proliferation[2][3]. HVEM/TNFRSF14 Protein, Human (164a.a, HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 164 amino acids (L39-V202) and is produced in HEK293 cells.

研究背景

HVEM 广泛表达于多种造血细胞,包括 B 细胞、T 细胞、NK 细胞、单核细胞和未成熟树突状细胞,以及多种非造血细胞和组织,包括肝、肾和肺[1].
人HVEM蛋白的氨基酸序列与小鼠HVEM蛋白的同源性较低。
HVEM被称为激活和抑制的“分子开关”模型。HVEM 提供抑制或激活信号并双向调节宿主免疫功能。HVEM与LIGHT或LIGHT-α结合发挥正向刺激作用,刺激淋巴细胞增殖、活化,诱发炎症反应;因此,为 T 细胞激活提供第二刺激信号。此外,HVEM与BTLA和CD160的结合会产生不利的调节作用,促进通过ERK1/2和PI3K(磷脂酰肌醇3激酶)-AKT(蛋白激酶B(PKB))途径的信号转导,导致IFNγ的产生,抑制 T 和 B 淋巴细胞活化和增殖以及 HVEM 与 HSV-gD 的结合,从而促进靶细胞中 HSV 的感染[2][3]
HVEM 被认为是免疫反应的分子开关,HVEM 诱导 DC 产生 IL-10,并显示出针对肌球蛋白引起的实验性自身免疫性心肌炎 (EAM) 的保护作用[4]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q92956 (L39-V202)

基因 ID
蛋白结构
N-term
HVEM (L39-V202)
Accession # Q92956
6*His
C-term
中文名
重组人 HVEM/TNFRSF14 蛋白 (164a.a, HEK293, His)
同用名
Tumor Necrosis Factor Receptor Superfamily Member 14; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; HveA; Tumor Necrosis Factor Receptor-Like 2; TR2; CD270; TNFRSF14; HVEA; HVEM
氨基酸序列

LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV

分子量

20-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HVEM/TNFRSF14 Protein, Human (164a.a, HEK293, His)
目录号:
HY-P70489
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