HVEM/TNFRSF14 蛋白, Human (Sf9, Fc)

HVEM (herpes virus entry mediator, TNFRSF14, CD270) is a member of the tumor necrosis factor receptor superfamily (TNFRSF). HVEM is a bidirectional molecular switch that transduces positive and negative signals. HVEM can deliver proinflammatory and survival signals when engaged by BTLA or LIGHT, stimulating lymphocyte proliferation, activation, and inducing inflammatory reactions. While, HVEM binds to CD160 and BTLA, inhibiting T- and B-lymphocyte activation and proliferation^[2][3]. HVEM/TNFRSF14 Protein, Human (Sf9, Fc) is a recombinant protein consisting of 146 amino acids (L39-K184) and is produced in Sf9 insect cells.

HVEM 广泛表达于多种造血细胞，包括 B 细胞、T 细胞、NK 细胞、单核细胞和未成熟树突状细胞，以及多种非造血细胞和组织，包括肝、肾和肺^[1]。
人 HVEM 蛋白的氨基酸序列与小鼠 HVEM 蛋白的同源性较低。
HVEM 被称为激活和抑制的“分子开关”模型。HVEM 提供抑制或激活信号并双向调节宿主免疫功能。HVEM 与 LIGHT 或 LIGHT-α 结合发挥正向刺激作用，刺激淋巴细胞增殖、活化，诱发炎症反应；因此，为 T 细胞激活提供第二刺激信号。此外，HVEM 与 BTLA 和 CD160 的结合会产生不利的调节作用，促进通过 ERK1/2 和 PI3K (磷脂酰肌醇 3 激酶)-AKT (蛋白激酶 B (PKB)) 途径的信号转导，导致 IFNγ 的产生，抑制 T 和 B 淋巴细胞活化和增殖以及 HVEM 与 HSV-gD 的结合，从而促进靶细胞中 HSV 的感染^[2][3]。
HVEM 被认为是免疫反应的分子开关，HVEM 诱导 DC 产生 IL-10，并显示出针对肌球蛋白引起的实验性自身免疫性心肌炎 (EAM) 的保护作用^[4]。

1.The ED₅₀ < 0.1 μg/ml, measured by the neutralization assay using 929 cells in presence of 0.25 ng/mL of human TNF-beta, corresponding to a specific activity of > 1.0 × 10⁴ units/mg.
2. Immobilized HVEM, hFc, Human at 2.0 μg/mL (100 µl/well) can bind biotinylated human BTLA with a linear range of 0.39-3.13 μg/mL.
3. Immobilized HVEM, hFc, Human at 2.0 μg/mL (100 µl/well) can bind biotinylated CD160, hFc, Human with a linear range of 0.39-3.13 μg/mL.

Human

Sf9 insect cells

C-hFc

Q92956 (L39-K184)

8764  [NCBI]

LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKRSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK

Approximately 45 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ma B, et al. High expression of HVEM is associated with improved prognosis in intrahepatic cholangiocarcinoma. Oncol Lett. 2021 Jan;21(1):69.  [Content Brief]

  [2]. Montgomery RI, et al. Herpes simplex virus-1 entry into cells mediated by a novel member of the TNF/NGF receptor family. Cell. 1996 Nov 1;87(3):427-36.  [Content Brief]

  [3]. Yu X, et al. BTLA/HVEM Signaling: Milestones in Research and Role in Chronic Hepatitis B Virus Infection. Front Immunol. 2019 Mar 29;10:617.  [Content Brief]

  [4]. Rodriguez-Barbosa JI, et al. HVEM, a cosignaling molecular switch, and its interactions with BTLA, CD160 and LIGHT. Cell Mol Immunol. 2019 Jul;16(7):679-682.  [Content Brief]

  [5]. Cai G, et al. Amelioration of myocarditis by HVEM-overexpressing dendritic cells through induction of IL-10-producing cells. Cardiovasc Res. 2009 Dec 1;84(3):425-33.  [Content Brief]