HVEM/TNFRSF14 蛋白, Human (Biotinylated, HEK293, Fc-Avi)

The HVEM/TNFRSF14 protein acts as a receptor for four ligands, including TNFSF14/LIGHT, LTA/lymphotoxin-α, BTLA, and CD160, forming a complex stimulatory and inhibitory signaling network. Utilizes the TRAF2-TRAF3 E3 ligase pathway to promote the survival and differentiation of immune cells. HVEM/TNFRSF14 Protein, Human (Biotinylated, HEK293, Fc-Avi) is the recombinant human-derived HVEM/TNFRSF14 protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.

HVEM/TNFRSF14 是四种不同配体的受体，错综复杂地协调着刺激和抑制信号通路的复杂网络。配体，包括 TNFSF14/LIGHT、同源三聚体 LTA/淋巴毒素-α 以及免疫球蛋白超家族成员 BTLA 和 CD160，共同定义了这个复杂的信号网络。HVEM/TNFRSF14 通过 TRAF2-TRAF3 E3 连接酶途径发挥作用，发出促进免疫细胞存活和分化的信号，在抗原呈递细胞和淋巴细胞之间的双向细胞-细胞接触信号传导中发挥关键作用。TNFSF14/LIGHT 连接后，HVEM/TNFRSF14 向 T 细胞传递共刺激信号，促进细胞增殖和效应功能。与 NK 细胞上的 CD160 相互作用可增强 IFNG 的产生和抗肿瘤免疫反应。在细菌感染中，HVEM/TNFRSF14 作为上皮细胞上皮内淋巴细胞 CD160 的信号传导受体，触发抗菌蛋白和促炎细胞因子的产生。此外，HVEM/TNFRSF14通过在活化的CD4+T细胞上结合CD160，下调CD28共刺激信号传导，限制记忆和同种抗原特异性免疫反应。HVEM/TNFRSF14 与 BTLA 表现出顺式和反式相互作用，在适应性免疫反应过程中的免疫调节和生存信号传导中发挥多种作用。此外，作为单纯疱疹病毒 1/HHV-1 的受体，HVEM/TNFRSF14 与微生物感染有关。

Immobilized Human LIGHT mFc at 2 μg/mL (100 μL/well) can bind Biotinylated Human HVEM Fc-Avi with a linear range of 0.6-39 ng/mL.

Human

HEK293

C-Avi;C-hFc

Q92956-1 (L39-V202)

8764  [NCBI]

Approximately 55-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of Tris with Glycine, Arginine and NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.