TFAM 蛋白, Human (His)

TFAM protein regulates lymphangiogenesis, interacts with TEK/TIE2 and ITGA5, and binds to the mitochondrial light chain promoter. It is part of the mitochondrial transcription initiation complex formed with TFB2M and POLRMT and is critical for accurate promoter recognition and transcription initiation. TFAM Protein, Human (His) is the recombinant human-derived TFAM protein, expressed by E. coli , with labeled tag. The total length of TFAM Protein, Human (His) is 204 a.a., with molecular weight of 28.5 kDa.

TFAM 蛋白参与淋巴管生成的调节，并与 TEK/TIE2 和 ITGA5 相互作用。它与线粒体轻链启动子结合，在线粒体转录调节中发挥作用。TFAM 与 TFB2M 和 POLRMT 一起是线粒体转录起始复合物的组成部分，是线粒体 DNA 基础转录所必需的。TFAM 将 POLRMT 募集至特定启动子，并诱导 POLRMT 的结构变化，以实现启动子打开和 DNA 非模板链捕获。它是线粒体 RNA 聚合酶准确识别启动子并促进转录起始所必需的。TFAM 可以解开 DNA 并通过其 HMG 盒将线粒体轻链启动子 DNA 弯曲成 U 形转弯形状。它对于维持线粒体 DNA 的正常水平至关重要，并且还可能在组织和压缩线粒体 DNA 中发挥作用。TFAM 可以作为单体结合 DNA，并可以形成同型二聚体。它在代谢应激下与 FOXO3、SIRT3 和 POLRMT 形成复合物，并与 TFB1M、TFB2M 和 CLPX 相互作用，增强 DNA 结合。

Human

E. coli

N-6*His

Q00059 (S43-C246)

7019

30 kDa

Greater than 95 % as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 100 mM Tris-HCl, 10 mM Sodium citrate, 2 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.