1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. hydB 蛋白, Pyrococcus furiosus (His)

hydB 蛋白, Pyrococcus furiosus (His)

目录号: HY-P701917
产品使用指南

HydB是双功能酶复合物的重要组成部分,是一种具有硫还原活性的NADPH依赖性析氢氢化酶。它有助于发酵生长过程中的氢循环,β和γ亚基催化细胞质从元素硫产生硫化氢。hydB 蛋白, Pyrococcus furiosus (His) 是重组的 hydB 蛋白,由 E. coli 表达,带有 N-6*His 标签。hydB 蛋白, Pyrococcus furiosus (His) 全长 367 个氨基酸。

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描述

HydB is an important component of the bifunctional enzyme complex and is a NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. It contributes to the hydrogen cycle during fermentative growth, with the beta and gamma subunits catalyzing the cytoplasmic production of hydrogen sulfide from elemental sulfur. hydB Protein, Pyrococcus furiosus (His) is the recombinant hydB protein, expressed by E. coli , with N-6*His labeled tag. The total length of hydB Protein, Pyrococcus furiosus (His) is 367 a.a., .

研究背景

HydB 是双功能酶复合物的组成部分,作为 NADPH 依赖性析氢氢化酶,具有硫还原活性。这种酶可能有助于发酵生长过程中的氢循环。值得注意的是,其活性并未通过 NAD 得到证实,并且 β 和 γ 亚基共同形成硫还原成分,负责在元素硫存在的情况下催化细胞质产生硫化氢。然而,当暴露于硫酸钠、亚硫酸钠、硫代硫酸钠或半胱氨酸时,HydB 就会失去活性。这些发现强调了它在氢代谢和硫还原的复杂过程中的作用,特别是在某些环境条件下。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

Q8U2E5 (M1-V367)

基因 ID

41712699

蛋白结构
N-term
6*His
hydB (M1-V367)
Accession # Q8U2E5
C-term
同用名
hydB; Sulfhydrogenase 1 subunit beta; Sulfhydrogenase I subunit beta; Sulfur reductase subunit HydB
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

hydB Protein, Pyrococcus furiosus (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
hydB Protein, Pyrococcus furiosus (His)
目录号:
HY-P701917
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