1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. Hydroxyacid oxidase 1 蛋白, Mouse (His)

Hydroxyacid oxidase 1 蛋白, Mouse (His)

目录号: HY-P71655
产品使用指南

羟基酸氧化酶 1 (HAO1) 是一种多功能 (S)-2-羟基酸氧化酶,对乙醇酸氧化具有强烈的偏好。这种酶促过程产生乙醛酸,乙醛酸是丙氨酸乙醛酸转氨酶合成甘氨酸的重要前体,可防止乙醛酸积累和肾结石形成。Hydroxyacid oxidase 1 蛋白, Mouse (His) 是重组的 羟基酸氧化酶 1 蛋白,由 E. coli 表达,带有 N-His 标签。Hydroxyacid oxidase 1 蛋白, Mouse (His) 全长 370 个氨基酸,分子量约为 ~45.0 kDa。

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描述

Hydroxy acid oxidase 1 (HAO1) is a multifunctional (S)-2-hydroxyacid oxidase with a strong preference for glycolate oxidation. This enzymatic process produces glyoxylic acid, an important precursor for the synthesis of glycine by alanine glyoxylate aminotransferase, which prevents glyoxylic acid accumulation and kidney stone formation. Hydroxyacid oxidase 1 Protein, Mouse (His) is the recombinant mouse-derived Hydroxyacid oxidase 1 protein, expressed by E. coli , with N-His labeled tag. The total length of Hydroxyacid oxidase 1 Protein, Mouse (His) is 370 a.a., with molecular weight of ~45.0 kDa.

研究背景

羟基酸氧化酶 1 (HAO1) 是一种多功能 (S)-2-羟基酸氧化酶,具有广泛的底物特异性,对乙醇酸氧化表现出明显的偏好。这种酶活性导致乙醛酸的产生,乙醛酸是丙氨酸-乙醛酸转氨酶过氧化物酶体合成甘氨酸所必需的关键前体。该途径在乙醛酸解毒、防止其积累和肾结石的潜在形成方面发挥着关键作用。HAO1 进一步显示出催化乙醛酸和长链羟基酸(例如 2-羟基十六酸和 2-羟基辛酸)氧化的能力。虽然人工电子受体 2,6-二氯苯酚靛酚 (DCIP) 在体外具有活性,但据信 O2 充当生理电子受体,导致 H2O2 的产生。HAO1 的多方面酶功能强调了其在各种代谢途径中的重要性。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

Q9WU19 (1M-370I)

基因 ID

15112  [NCBI]

蛋白结构
N-term
His
Hydroxyacid oxidase 1 (1M-370I)
Accession # Q9WU19
C-term
同用名
Hao1; Gox1; Hao-1; Hydroxyacid oxidase 1; HAOX1; EC 1.1.3.15; Glycolate oxidase; GOX
氨基酸序列

MLPRLVCISDYEQHVRSVLQKSVYDYYRSGANDQETLADNIQAFSRWKLYPRMLRNVADIDLSTSVLGQRVSMPICVGATAMQCMAHVDGELATVRACQTMGTGMMLSSWATSSIEEVAEAGPEALRWMQLYIYKDREISRQIVKRAEKQGYKAIFVTVDTPYLGNRIDDVRNRFKLPPQLRMKNFETNDLAFSPKGNFGDNSGLAEYVAQAIDPSLSWDDITWLRRLTSLPIVVKGILRGDDAKEAVKHGVDGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIIWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI

分子量

Approximately 45.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Hydroxyacid oxidase 1 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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