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  4. IBSP 蛋白, Human (His-SUMO)

IBSP 蛋白, Human (His-SUMO)

目录号: HY-P72241
产品使用指南

IBSP 蛋白对羟基磷灰石具有很强的亲和力，在矿化基质形成中发挥着至关重要的作用，表明了其在这方面的整体性质。它的紧密结合表明在细胞-基质相互作用中发挥重要作用，特别是在促进 RGD 依赖性细胞附着方面。IBSP 蛋白, Human (His-SUMO) 是重组的 IBSP 蛋白，由 E. coli 表达，带有 N-6*His, N-SUMO 标签。IBSP 蛋白, Human (His-SUMO) 全长 153 个氨基酸，分子量约为 ~32.4 kDa。

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描述

The IBSP protein has a strong affinity for hydroxyapatite and plays a crucial role in mineralized matrix formation, indicating its overall nature in this regard. Its tight binding suggests an important role in cell-matrix interactions, particularly in promoting RGD-dependent cell attachment. IBSP Protein, Human (His-SUMO) is the recombinant human-derived IBSP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of IBSP Protein, Human (His-SUMO) is 153 a.a., with molecular weight of ~32.4 kDa.
研究背景

IBSP 蛋白对羟基磷灰石表现出很强的结合亲和力，表明在矿化基质的形成中起着至关重要的作用。它可能是该基质的一个组成部分，这意味着它在细胞-基质相互作用中的重要性。值得注意的是，IBSP 蛋白在促进 Arg-Gly-Asp (RGD) 依赖性细胞附着中发挥关键作用，强调其参与细胞粘附过程。观察到的与羟基磷灰石的紧密结合及其与矿化基质的明显整合强调了 IBSP 在生物系统内细胞-基质相互作用的结构和功能方面的重要性。
种属

Human
表达系统

E. coli
标签

N-6*His;N-SUMO
蛋白编号

P21815 (A129-E281)
基因 ID
蛋白结构
N-term
6*His-SUMO
IBSP (A129-E281)
Accession # P21815
C-term
同用名
BNSP; Bone sialoprotein 2; Bone sialoprotein II; BSP; BSP II; BSPII; Cell binding sialoprotein; Cell-binding sialoprotein; IBSP; Integrin binding sialoprotein; Integrin-binding sialoprotein; SIAL_HUMAN; SPII
氨基酸序列

AIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESE

分子量

Approximately 32.4 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

IBSP Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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