ICAM-1/CD54 蛋白, Mouse (HEK293, His)

ICAM-1/CD54 protein is a key player in cellular interactions, acting as a ligand for LFA-1. It facilitates endothelial cup assembly during leukocyte migration and regulates immune responses. ICAM-1 also interacts with MUC1, promoting cell aggregation, and participates in immunological synapse formation between antigen-presenting cells and T cells. These interactions showcase ICAM-1's diverse role in cellular adhesion and immune modulation. ICAM-1/CD54 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag. The total length of ICAM-1/CD54 Protein, Mouse (HEK293, His) is 458 a.a., with molecular weight of 80-90 kDa.

ICAM-1/CD54 蛋白作为介导细胞相互作用的关键成分而出现，充当白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）的配体。值得注意的是，在白细胞跨内皮迁移过程中，ICAM-1 的参与在通过 ARHGEF26/SGEF 和 RHOG 激活促进内皮顶杯组装方面发挥着至关重要的作用。这种复杂的分子过程与 ICAM 家族的其他成员相似，强调了 ICAM-1 参与调节免疫反应和炎症反应。除了在白细胞粘附中的作用外，ICAM-1 还作为同源二聚体发挥作用，并与 MUC1 相互作用，促进上皮细胞中的细胞聚集。此外，ICAM-1 与 ARHGEF26/SGEF 建立相互作用，并与 T 细胞侧的 CD81、CD247 和 CD9 相关联，有助于抗原呈递细胞和 T 细胞之间免疫突触的形成。这些多方面的相互作用凸显了 ICAM-1 在细胞粘附和免疫调节中的多种功能。

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/mL, 100 μL/well ),approximately > 40% cells will adhere specifically.

Mouse

HEK293

C-His

P13597 (Q28-N485)

15894  [NCBI]

MASTRAKPTLPLLLALVTVVIPGPGDAQVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNN

80-90 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.