ICAM-1/CD54 蛋白, Rat (HEK293, His)

ICAM-1/CD54 protein is a crucial ligand for leukocyte adhesion, promoting endothelial apical cup assembly during migration.It regulates immune responses and inflammation.ICAM-1 also interacts with MUC1, promoting cell aggregation, and contributes to immunological synapse formation between antigen-presenting cells and T cells.These interactions showcase ICAM-1's diverse functions in cellular adhesion and immune modulation.ICAM-1/CD54 Protein, Rat (HEK293, His) is the recombinant rat-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-His labeled tag.

ICAM-1/CD54 蛋白是白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）的关键配体，在多种细胞相互作用中发挥着关键作用。具体而言，在白细胞跨内皮迁移过程中，ICAM-1 的参与通过 ARHGEF26/SGEF 和 RHOG 的激活促进内皮顶杯的组装。这种复杂的分子过程让人想起其他 ICAM 家族成员，强调了 ICAM-1 参与调节免疫反应和炎症反应。除了在白细胞粘附中的作用外，ICAM-1 还形成同二聚体并与 MUC1 相互作用，促进上皮细胞中的细胞聚集。此外，ICAM-1 与 ARHGEF26/SGEF 结合，并在 T 细胞侧与 CD81、CD247 和 CD9 相互作用，有助于在抗原呈递细胞和 T 细胞之间建立免疫突触。这些多功能的相互作用凸显了 ICAM-1 在细胞粘附和免疫调节中的多方面功能。

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to rat ICAM1 coated plates (12.5 µg/mL, 100 µL/well), approximately > 30% cells will adhere specifically.

Rat

HEK293

C-His

Q00238 (Q28-T493)

25464  [NCBI]

Partial

70-75 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.