ICAM-5 蛋白, Human (HEK293, His)

The ICAM-5 protein functions as a ligand for the leukocyte adhesion protein LFA-1 (integrin α-L/β-2) and mediates the cell adhesion process. As a member of the ICAM family, ICAM-5 interacts with LFA-1 and is critical for immune cell adhesion and communication. ICAM-5 Protein, Human (HEK293, His) is the recombinant human-derived ICAM-5 protein, expressed by HEK293 , with C-His labeled tag. The total length of ICAM-5 Protein, Human (HEK293, His) is 803 a.a., with molecular weight of ~87.2 kDa.

ICAM-5 蛋白作为白细胞粘附蛋白 LFA-1（整合素 α-L/β-2）的配体，表明其在介导细胞粘附过程中的作用。作为 ICAM 蛋白家族的一员，ICAM-5 与 LFA-1 相互作用有关，LFA-1 对于免疫细胞之间的粘附和通讯至关重要。这种相互作用可能在免疫反应和细胞运输中发挥作用，有助于免疫细胞活动的动态调节。ICAM-5 与 LFA-1 的结合强调了其在调节免疫细胞相互作用和功能的复杂粘附分子网络中的重要性。

Measured by its ability of the immobilized protein to support the adhesion of PMA-stimulated Jurkat cells. When 5×10⁴ cells/well are added to ICAM-5 coated plates (25 μg/mL,100 μL/well) in the presence of 10 ng/mL PMA, approximately 42.83% will adhere after 2 hour at 37°C.

Human

HEK293

C-His

Q9UMF0 (E32-P834)

7087  [NCBI]

EPFWADLQPRVAFVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELMPLPPWQPVGENFTLSCRVPGAGPRASLTLTLLRGAQELIRRSFAGEPPRARGAVLTATVLARREDHGANFSCRAELDLRPHGLGLFENSSAPRELRTFSLSPDAPRLAAPRLLEVGSERPVSCTLDGLFPASEARVYLALGDQNLSPDVTLEGDAFVATATATASAEQEGARQLVCNVTLGGENRETRENVTIYSFPAPLLTLSEPSVSEGQMVTVTCAAGAQALVTLEGVPAAVPGQPAQLQLNATENDDRRSFFCDATLDVDGETLIKNRSAELRVLYAPRLDDSDCPRSWTWPEGPEQTLRCEARGNPEPSVHCARSDGGAVLALGLLGPVTRALSGTYRCKAANDQGEAVKDVTLTVEYAPALDSVGCPERITWLEGTEASLSCVAHGVPPPDVICVRSGELGAVIEGLLRVAREHAGTYRCEATNPRGSAAKNVAVTVEYGPRFEEPSCPSNWTWVEGSGRLFSCEVDGKPQPSVKCVGSGGATEGVLLPLAPPDPSPRAPRIPRVLAPGIYVCNATNRHGSVAKTVVVSAESPPEMDESTCPSHQTWLEGAEASALACAARGRPSPGVRCSREGIPWPEQQRVSREDAGTYHCVATNAHGTDSRTVTVGVEYRPVVAELAASPPGGVRPGGNFTLTCRAEAWPPAQISWRAPPGALNIGLSSNNSTLSVAGAMGSHGGEYECAATNAHGRHARRITVRVAGP

Approximately 112 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.