ICOS 蛋白, Rat (HEK293, Fc)

ICOS is a key protein for T cell function and can significantly enhance various T cell responses to foreign antigens. It plays a crucial role in promoting T cell proliferation, lymphokine secretion and upregulation of intercellular interaction molecules, and promoting efficient secretion of B cell antibodies. ICOS Protein, Rat (HEK293, Fc) is the recombinant rat-derived ICOS protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ICOS Protein, Rat (HEK293, Fc) is 122 a.a., with molecular weight of 43-47 kDa.

ICOS 是 T 细胞功能中必需的蛋白质，可显着增强 T 细胞对外来抗原的各种反应。它在促进T细胞增殖、分泌淋巴因子、上调细胞间相互作用分子以及有效协助B细胞抗体分泌等方面发挥着关键作用。值得注意的是，ICOS 对于 T 细胞依赖性抗原的正常抗体反应是不可或缺的，可促进 T 细胞和 B 细胞之间的相互作用。有趣的是，ICOS 不会提高白细胞介素 2 的产生，但会促进白细胞介素 10 的合成。此外，它在防止预激活 T 细胞凋亡方面发挥着至关重要的作用，并在 CD40 介导的免疫球蛋白同种型类型转换中发挥重要作用。该蛋白质通过二硫键形成同二聚体，在调节免疫反应中发挥多方面的作用。

Measured by its ability to inhibit Jurkat proliferation induced by B7-H2 in the presence of anti-CD3. The ED₅₀ for this effect is 3.252 µg/mL in the presence of 3 µg/mL Human B7‑H2 and 20 ng/mL of anti-CD3, corresponding to a specific activity is 307.503 units/mg.

Rat

HEK293

C-hFc

Q9R1T7 (E21-L142)

64545  [NCBI]

ELNDLANHRMFSFHDGGVQISCNYPETVQQLKMQLFKDREVLCDLTKTKGSGNTVSIKNPMSCPYQLSNNSVSFFLDNADSSQGSYFLCSLSIFDPPPFQEKNLSGGYLLIYESQLCCQLKL

Approximately 43-50 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.