IdeS 蛋白, Streptococcus pyogenes (His)

IdeS Protein is a highly specific IgG endopeptidase evolved from Streptococcus pyogenes, which can degrade IgG and participate in the immune response. IdeS Protein inhibits the function of certain neutrophil effectors, namely the production of reactive oxygen species (ROS), independently of IgG endopeptidase activity. IdeS Protein, Streptococcus pyogenes (His) is the recombinant IdeS protein, expressed by E. coli , with N-10*His labeled tag. The total length of IdeS Protein, Streptococcus pyogenes (His) is 312 a.a., with molecular weight of ~36.2 kDa.

IdeS 是由化脓性链球菌进化出一种高度特异性的 IgG 内肽酶，它不仅可以降解 IgG，还可以直接或间接地抑制先天免疫反应，从而促进链球菌在炎症环境中的生存。IdeS 被发现与链球菌的 Mac-1 相同，Mac-1 是一种被认为通过抑制 Fc 受体 (CD16) 对 IgG 和/或补体配置的识别来抑制吞噬的蛋白。IdeS/Mac-1可以抑制某些中性粒细胞效应物的功能，即活性氧 (ROS) 的产生，而不依赖于 IgG 内肽酶的活性。

One unit digests ≥ 95% of 1µg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.

Others

E. coli

N-10*His

F8V4V0 (D30-N341)

/

Approximately 36.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of PBS, 50 % Glycerol, pH 6.6.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.