IFIH1 蛋白, Human (His)

The IFIH1 protein is an innate immune receptor that detects viral nucleic acids and triggers an antiviral response. IFIH1 is critical for type I interferon induction and recognizes ligands such as mRNA lacking 2'-O-methylation and long double-stranded RNA. IFIH1 Protein, Human (His) is the recombinant human-derived IFIH1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IFIH1 Protein, Human (His) is 326 a.a., with molecular weight of ~41.5 kDa.

IFIH1蛋白作为一种先天免疫受体，充当病毒核酸的细胞质传感器，在检测病毒感染和启动级联抗病毒反应中发挥着关键作用。它对于诱导 I 型干扰素和促炎细胞因子响应病毒刺激至关重要。IFIH1 识别配体，例如 5' 帽缺乏 2'-O-甲基化的 mRNA 和长双链 RNA (>1 kb)。配体结合后，它与线粒体抗病毒信号蛋白 (MAVS/IPS1) 结合，激活 IKK 相关激酶 TBK1 和 IKBKE，磷酸化干扰素调节因子（IRF3 和 IRF7），导致抗病毒免疫基因（包括干扰素 IFN）的转录-α和IFN-β。该蛋白负责检测各种病毒，包括小核糖核酸病毒科的成员，如脑心肌炎病毒 (EMCV)、门戈脑心肌炎病毒 (ENMG)、鼻病毒和 SARS-CoV-2。IFIH1 还参与针对具有双链 DNA 基因组的病毒（如牛痘病毒）的抗病毒信号传导。此外，它通过识别核糖核酸酶 L (RNase L) 病毒感染期间产生的 RNA 代谢物，有助于先天免疫信号放大。此外，IFIH1 可能增强自然杀伤细胞功能并参与各种肿瘤细胞系的生长抑制和凋亡。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9BYX4 (K700-D1025)

64135  [NCBI]

KLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED

Approximately 41.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.