1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-alpha
  5. IFN-alpha 2a
  6. IFN-alpha 2/IFNA2 蛋白, Human (P.pastoris)

IFN-alpha 2/IFNA2 蛋白, Human (P.pastoris)

目录号: HY-P73246
COA 产品使用指南

IFN-alpha 2 (IFNA2) 属于 I 型干扰素家族,是病毒感染的细胞分泌的一种蛋白质,作用于其他细胞并抑制病毒感染。IFN-alpha 2 对 CD8+ T 细胞具有细胞毒性,能够诱导 CD4+ T 细胞耗损增加。重组人 IFN-alpha 2/IFNA2 蛋白 (P.pastoris) (C24-E188) 全长 165 个氨基酸,由酵母菌株 P. pastoris 产生,不带标签。

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10 μg ¥850 In-stock
50 μg ¥2100 In-stock
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250 μg   询价  

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描述

IFN-alpha 2 (IFNA2), belongs to type I interferon family, is a protein secreted by cells infected by a virus and acting on other cells to inhibit viral infection[1]. IFN-alpha 2 exerts cytotoxic activity against CD8+ T cells and induces CD4+ T cell depletion[3]. IFN-alpha 2/IFNA2 Protein, Human (P.pastoris) contains 165 a.a., is produced in yeast strain P. pastoris with tag free.

研究背景

IFN-alpha 2 (IFNA2; IFN-α2), belongs to the type I interferon family, produced by the plasmacytoid dendritic cells (pDCs) exposure to HIV-1BaL in order to inhibit viral infection[1].
Interferon (IFN) is originally identified as a substance ‘interfering’ with viral replication in vitro. IFN-α/β and related molecules are classified as type I IFNs, as for the other two types of type II IFN (IFN-γ) and type III IFNs (IFN-λ), respectively[2].
IFN-alpha 2 subtype is the only one that is currently licensed to treat infections caused by hepatitis B virus (HBV) and HCV[3].
IFN-alpha 2 shows a Sortilin-dependent trafficking in cells and increases the expression level of interferon-stimulated genes (ISGs) in HIV-infected cells[1][4]. It also exhibits cytotoxic activity against CD8+ T cells and enhances CD4+ T cell depletion[3].
Among the IFN-alpha 2 alleles, IFN-alpha 2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant[5].
IFN-alpha 2 has a bored application in research of cancer, including some hematological malignancies and solid tumors[6]. As for a wildly use of IFN in animal disease model, the sequence of amino acids in IFNA2a protein of human is very different from mouse (59.57%).

体内研究
(In Vivo)

IFNA2a (hydrodynamic injection with plasmids) leads to upregulation of interferon-stimulated genes (ISGs) and results HIV-1 induced CD4+ T cell depletion in the hu-PBL humanized mouse model[3].

生物活性

Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV) and the EC50 is typically 0.6-4.2 pg/mL.

种属

Human

表达系统

P. pastoris

标签

Tag Free

蛋白编号

P01563 (C24-E188)

基因 ID

3440  [NCBI]

中文名
重组人 IFN-alpha 2/IFNA2 蛋白 (P.pastoris)
同用名
Interferon alpha-2; IFN-alpha-2; LeIF A; IFNA2; IFNA2A
氨基酸序列

CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE

分子量

Approximately 19.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Solution.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

IFN-alpha 2/IFNA2 Protein, Human (P.pastoris) 相关分类

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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