IFN-beta 蛋白, Mouse (HEK293, Fc)

IFN-β protein is a type I interferon that plays a key role in the innate immune response by binding to IFNAR2 and IFNAR1 receptors and activating Jak-STAT signaling. This results in transcriptional regulation of interferon-regulated genes, affecting antiviral proteins, cell proliferation regulators, and immunomodulatory proteins. IFN-beta Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived IFN-beta protein, expressed by HEK293 , with C-hFc labeled tag. The total length of IFN-beta Protein, Mouse (HEK293, Fc) is 182 a.a., with molecular weight of ~58 kDa.

IFN-β 蛋白是一种 I 型干扰素细胞因子，在针对感染、肿瘤和各种炎症刺激的先天免疫反应中发挥着关键作用。其信号传导涉及与高亲和力受体 IFNAR2 和低亲和力受体 IFNAR1 结合，形成异二聚体复合物并激活经典的 Jak-STAT 信号传导途径。这种激活导致干扰素调节基因的转录调节，包括抗病毒蛋白、细胞增殖和分化的调节因子以及免疫调节蛋白。虽然主要通过 IFNAR1-IFNAR2 异二聚体受体发出信号，但 IFN-β 也可以单独与 IFNAR1 一起发挥作用，独立于 Jak-STAT 途径发挥作用。IFN-β 引发多种反应，包括抗病毒和抗菌活性，并影响 B 细胞发育、骨髓生成和脂多糖 (LPS) 诱导的肿瘤坏死因子的产生。除了其免疫功能外，IFN-β 通过调节多巴胺更新和保护多巴胺能神经元、促进神经元自噬、促进 α-突触核蛋白清除，从而防止多巴胺能神经元丢失，在神经元稳态中发挥着至关重要的作用。值得注意的是，IFN-β 在诱导细胞凋亡和抗增殖途径方面比干扰素 α (IFN-α) 表现出更强的效力，这对控制肿瘤细胞生长至关重要。它在这些监管过程中充当单体。

Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV) and the ED₅₀ is typically 8 pg/mL.

Mouse

HEK293

C-hFc

P01575 (M1-N182)

15977  [NCBI]

Approximately 58 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.