1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-gamma Receptor
  5. IFN-gamma R1
  6. IFN-gamma R1/CD119 蛋白, Mouse (HEK293, Fc)

IFN-gamma R1/CD119 蛋白, Mouse (HEK293, Fc)

目录号: HY-P73133
产品使用指南

IFN-gamma R1,IFN-gamma 受体的亚基之一,是 IFN-γ 的结合受体。IFN-gamma R1 可以与 IFN-gamma R2 结合形成功能性受体。与配体 IFN-γ 结合后,IFN-gamma R1 和 IFN-gamma R2 寡聚化并发生磷酸化。然后,JAK1 和 JAK2 发生磷酸化并激活,STAT1 被募集到受体复合物附近。磷酸化的 STAT1 易位至细胞核,从而调节 IFN-反应性的基因 (例如 CD54) 的表达。FN-gamma R1/CD119 蛋白, Mouse (HEK293, Fc) 是一种由 HEK293 细胞产生的重组小鼠 IFN-gamma R1 (M1-D253),C 端带有 hFc 标签。

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描述

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)[2]. IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) is a recombinant mouse IFN-gamma R1 (M1-D253) with C-terminal hFc tag, which is produced in HEK293 cells.

研究背景

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 is constitutively expressed on the surface of almost all cells[1].
IFN-gamma R1 can associate with IFN-gamma R2 to form a functional receptor. Upon binding with IFN-gamma, IFNγR1 and IFNγR2 oligomerize and transphosphorylate[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. The phosphorylation of IFNγR1 creates a docking site for STAT1 and leads to the phosphorylation of STAT1. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54). IFN-gamma R1 deficiencies are associated with immune responses mediated by IFN-γ, and increased susceptibility to infections. IFN-gamma R1 signaling pathway is important in activating cancer cell death and inhibiting cancer progression[3]
Mouse IFN-gamma R1 consists of extracellular domain (A26-S254), helical domain (I255-Y275), and cytoplasmic domain (W276-S477). The sequence of amino acids in IFNAR1 differs in different species. Mouse IFN-gamma R1 shares 50% aa sequence identity with human. IFN-gamma R1 plays a critical role in antimicrobial, antiviral, and antitumor responses[2].

体外研究
(In Vitro)

IFN-gamma R1 (murine, 0-20 μg/mL, 4 h) suppresses AP(+)-exosome-mediated NO synthesis in RAW264.7 cells[4].

生物活性

Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His at 10 μg/mL (100 μl/well) can bind IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) and the EC50 is 58.2-135.9 ng/mL.

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

P15261 (M1-D253)

基因 ID

15979  [NCBI]

中文名
重组小鼠 IFN-gamma R1/CD119 蛋白 (HEK293, Fc)
同用名
Interferon gamma receptor 1; IFN-gamma-R1; CD119; Ifngr1; Ifngr
分子量

70-75 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IFN-gamma R1/CD119 Protein, Mouse (HEK293, Fc) 相关分类

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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