IGFBP-4 蛋白, Human (HEK293, C-His)

IGFBP-4 Protein, Human is a member of the insulin-like growth factor-binding protein (IGFBP) family, binds to insulin-like growth factors, mediates IGF-independent biologic activities.

胰岛素样生长因子结合蛋白 (IGFBP) 家族由六种不同的蛋白成员 (IGFBP1-6) 组成，对 IGF-1 和 IGF-2 表现出高度抑制作用。IGFBP-4 是最小的成员，通过与 IGF 结合来调节生物功能。此外，IGFBP-4 介导不依赖于 IGF 的生物活性。IGFBP-4 抑制结直肠癌、乳腺癌和胶质母细胞瘤细胞的生长。IGFBP-4 在严重急性呼吸窘迫综合征的发生中发挥重要的炎症介质作用，与癌症的增殖、迁移和凋亡有关^[1]。

1.The ED₅₀ is <50 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15.0 ng/ml human IGF-II.
2. Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells.The ED₅₀ is 0.07882-0.1768 μg/mL in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is > 5656.1 units/mg

• 
  Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1768μg/ml in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 5656.1 units/mg.

Human

HEK293

C-6*His

P22692 (D22-E258)

3487  [NCBI]

DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE

approximately 34.17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Li W, et al. Insulin-like growth factor binding protein-4 inhibits cell growth, migration and invasion, and downregulates COX-2 expression in A549 lung cancer cells. Cell Biol Int. 2017 Apr;41(4):384-391.  [Content Brief]