IGFBP-1 蛋白, Canine (HEK293, His)

IGFBP-1 Protein is a member of the insulin-like growth factor binding protein (IGFBP) family that binds insulin-like growth factor (IGFs) I and II to extend the half-life of IGFs. IGFBP-1 inhibits proliferation, invasion, migration and apoptosis of HTR-8/SVneo cells by inducing MMP-26 expression. IGFBP1 may be a new prognostic factor and a target of molecular targeted therapy for gastric cancer. IGFBP-1 Protein, Canine (HEK293, His) is the recombinant canine-derived IGFBP-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-1 Protein, Canine (HEK293, His) is 224 a.a., with molecular weight of ~27 kDa.

胰岛素样生长因子结合蛋白 1 (Insulin-like growth factor-binding protein 1, IBP-1) 又称胎盘蛋白 12 (placental protein 12, PP12)，是一种由 IGFBP1 基因编码的蛋白。IGFBP-1 是胰岛素样生长因子结合蛋白 (IGFBP) 家族的成员，可结合胰岛素样生长因子 (IGFs) I 和 II，并在血浆中循环。IGFBP-1 蛋白的结合延长了 IGFs 的半衰期，并改变了它们与细胞表面受体的相互作用。IGFBP-1 促进细胞迁移的能力，对 IGF1 和 IGF2 具有相同的结合亲和力。IGFBP-1 通过诱导 MMP-26 表达抑制 HTR-8/SVneo 细胞的增殖、侵袭、迁移和凋亡。IGFBP1 可能成为胃癌新的预后因子和分子靶向治疗的靶点。

Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED ₅₀ this effect is 0.5299 µg/mL, corresponding to a specific activity is 1.887×10³ U/mg.

Canine

HEK293

C-6*His

XP_005629556.1 (T26-S249)

480812  [NCBI]

TPQPWHCAPCTPEKLALCPPVPASCAETALPAGCGCCPMCALPQDAPCGVATARCATGLSCRAAPGEERPLHALIRGRGVCVPTDPAADAKESSESSEITQEQLLENFHLMVPSEEDTPILWNAVRNYKTVRSDDSDKLKEPCRRELHEVLARLAEEQASRQLYSFYLPNCNKNGFYHSRQCKTAMDGQRGLCWCVYPWNGERIPGSVEVRGDPNCGQYFTGHS

Approximately 27 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.